Transcript Phylogenetic analysis of flatfish species (Teleostei

Phylogenetic analysis of flatfish
species (Teleostei, Pleuronectiformes)
based on cytochrome oxidase 1 (Co-1)
and cytochrome b (Cyt-b) genes
Sharina S.N., Kartavtsev Y.P.
INTRODUCTION
 The phylogenetic relationships within the
order are still problematic
 Fish mitochondrial DNA is now widely used
with phylogenetic purposes
 Most popular in phylogenetics are
sequences of cytochrome b (Cyt-b) and
cytochrome oxidase 1 (Cо-1) genes, which
used for taxa comparison at the speciesfamily level.
Aim:
Molecular phylogenetic investigation of
relationships among order Pleuronectiformes
 To make reconstruction the phylogenetic
relationships based on Co-1 and Cyt-b
gene sequencing among some flatfish
species were made.
 To confirm or disprove the monophyly of
the flatfish family Pleuronectidae and
genus Pleuronectes several tree types
were built.
Materials and methods
Samples of the collection
Kievka Bay
Vostok Bay
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(Kartavtsev et al., 2007)
Flatfish-cytb-F – ATGGCCAACCTCCGTAAATCCCACCCCCTTC
Flatfish-cytb-R – CTGGGGCTCTGGACGCTGAGCTACTAGTGC
(~1441 bp)
20 spesies of Pleuronectiformes
for Co-1 gene
47 species of Pleuronectiformes
for Cyt-b gene
2 species of Gadiformes as
outgroup
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Flatfish-1-F
Flatfish-1-R
Flatfish-2-F
Flatfish-2-R
TCTTTAGATTTGCAATCTAACATGTA
GTCAGTAGCATTGTAATTCCTGCGGC
TGGGCCCATCACATATTTACAGTCGG
CAGAGCGGTTATGTGGTTGGCTTGAA
(~1500 bp)
Rooted consensus (50%)
MP-tree showing
phylogenetic
interrelationships on the
basis of Co-1 sequence data.
In the nodes bootstrap
support (n=1000) for MP and
NJ (MP/NJ) are shown. The
tree was rooted with the
sequences of two out-group
species: Gadiformes.
Rooted consensus (50%)
BA-tree showing
phylogenetic
interrelationships on the
basis of Cyt-b sequence data.
In the nodes bootstrap
support (n=1000) for NJ and
MP and repetition
frequencies for n=106
simulated generations for BA
(BA/MP/NJ) are shown. The
tree was built based on the
TrN+I+G model and was
rooted with the sequences of
two out-group species:
Gadiformes.
Hippoglossinae
Hippoglossoidinae
Pleuronectinae
Conclusions:
 Family Pleuronectidae with high confidence
may be considered as monophyletic.
 According the last systematical revision, we
have sampled in the genus Pleuronectes only
one species P. pinnifasciatus. Other species,
placed in different genera Pseudopleuronectes,
Liopsetta, and Lepidopsetta.
 The diagnostics of species (barcoding) based
on Co-1 and Cyt- b gene sequences is highly
effective because of low intraspecies and high
interspecies variability of this markers.
THANKS FOR ATTENTION!