Transcript Lecture10

BIS101-003
Office hours/review sessions for Midterm I
Midterm I - Tuesday, October 21
Office hours/review sessions:
R Oct 16 1-2pm 145ABriggs Rima
F Oct 17 12-1pm 3061SLB Engebrecht
Su Oct 19 6-8pm 2205Haring Engebrecht
M Oct 20 12-1pm 3061SLB Rebecca
No calculators or cell phones during test, use pen if you want the
possibility to request a regrade
Review Quiz
Scores: 291
Missing:2
High: 100.00%
Low: 0.00%
Median:95.00%
Mean: 92.43%
Standard Deviation:9.95
Critical Genetic Link Found Between Human Taste
Differences And Nicotine Dependence
http://www.sciencedaily.com/releases/2008/10/081014111301.htm
Two interacting genes related to bitter taste sensitivity,
TAS2R16 and TAS2R38
Tiny Worm Provides Model For The Genetics Of
Nicotine Dependence
http://www.sciencedaily.com/releases/2006/11/061103083610.htm
CQ1
A cross is made in E. coli between an Hfr strain that is leu+ his+
gly+ Strs and an F- strain that is leu- his- gly- StrR. Interrupted
mating studies show that his+ enters the recipient last. In an interrupted
mating between the same two strains, what medium would you select the
conjugates on before screening for recombinants?
A.
B.
C.
D.
Medium containing his and lacking streptomycin
Medium containing his and containing streptomycin
Medium lacking his and containing streptomycin
Medium lacking his and lacking streptomycin
CQ2
You select on medium lacking his and containing streptomycin and
then test by replica plating for the presence of the leu+ and gly+ alleles.
The following numbers of individuals are found for each genotype:
gly+ leu+ his+ =
259
gly- leu+ his+ =
1
gly+ leu- his+ =
11
gly- leu- his+ =
29
300
What is the gene order?
A. gly leu his
B. leu gly his
C. gly his leu
CQ3
You select on medium lacking his and containing streptomycin and
then test by replica plating for the presence of the leu+ and gly+ alleles.
The following numbers of individuals are found for each genotype:
gly+ leu+ his+ =
259
gly- leu+ his+ =
1
leu gly his
gly+ leu- his+ =
11
gly- leu- his+ =
29
300
What are the map distances between these three genes?
A. leu 4mu gly 10mu his
B. leu 10mu gly 4mu his
C. leu 10mu gly
10mu his
T4 bacteriophage
CQ4
In a generalized transduction experiment, the T1 phage particles
growing on E. coli with the genotype val+ ala+ trp+ are collected and
allowed to infect E. coli cells with the genotype val- ala- trp-. The
cotransduction frequencies are calculated for the different gene
combinations: ala-trp = 0.2
ala-val = 0.8
val-trp = 0.6
The gene order is:
A. ala - trp - val
B. ala - val - trp
C. val - ala - trp