Transcript EXCELMEAT: An International Research Network for - BDPorc

EXCELMEAT
An International Research Network for
the Genetic Improvement of Pigmeat
Quality and the Development of
Biosensing Technologies as Rapid and
Cost-Effective Tools for Meat Quality
Evaluation
FP7-PEOPLE-2009-IRSES
Dr Jules Hernández
EXCELMEAT coordinator
IRTA Monells, March 2012
INDEX
1.
What is EXCELMEAT?
2.
Pork Quality Views
3.
Partners
4.
Work Packages
5.
Genes and meat quality
6.
Workshop
7.
Potential EU project?
WHAT IS EXCELMEAT?
• EU funded scientific network
• It funds travel + accommodation
• It does not fund research costs
• Opportunity to establish international
collaborations
• It could lead to a European Project
• www.bdporc.irta.es/excelmeat/index.html
PORK QUALITY:
THE CONSUMER
01.1 QUÈ ÉS?
PORK QUALITY:
THE SCIENTIST
01.1 QUÈ ÉS?
ROLE OF
GENETICS
Halothene Gene
01.1 QUÈ ÉS?
ROLE OF
GENETICS
Acid Meat Gene
01.1 QUÈ ÉS?
ROLE OF
GENETICS
• Genetics
gives raw material
01.1 QUÈ ÉS?
• No environmental change will
transform Pietrain into Duroc
• No simple Mendelian explanation
for most complex traits
• The way: Genetics x Environment
PARTNERS
01.1 QUÈ ÉS?
1.
IRTA
2.
Universitat de Lleida (UdL)
3.
University of Bologna (UNIBO)
4.
University of West England (UWE)
5.
Ukranian Institute of Pig Breeding (IPBUA)
6.
Chinese Academy of Agricultural Sciences (CAAS)
7.
Katholieke Universiteit Leuven (KU Leuven)
8.
Iowa State University (ISU)
TÍTOL DEL
POWER POINT.
PRESENTACIÓ
WORK PACKAGES
1.
Pleiotropic and epigenetic studies of meat quality markers
2.
Muscle growth and development
3.
Regulation of fat deposition and fatty acid composition
4.
Animal welfare and handling as a source of stressors that
affect meat quality
5.
Development of novel technology for rapid and inexpensive
evaluations of meat quality parameters
TÍTOL DEL
POWER POINT.
PRESENTACIÓ
WP1
PLEIOTROPY AND EPIGENETICS
• Pleiotropy
 One gene affecting 2+ traits
 Good genes for meat quality should not be bad for
production/survival
 Data: >100 traits (fat, growth, sensorial) in Duroc (project
LIPEXQ, Dr. Quintanilla)
• Epigenetics
 How DNA methylation controls gene expression?
 Data: gene HMGA1 in Ukranian pigs + Duroc
TÍTOL DEL
POWER POINT.
PRESENTACIÓ
WP2
MUSCLE GROWTH AND
DEVELOPMENT
• Meat tenderness
 Calpain (proteolysis)
 Calpastatin (calpain inhibitors)
 Cathepsin D (proteolisis)
 Cystatins (cathepsin inhibitors)
 Myopalladin (Z-line and gene expression regulation)
 Myostatin (stops muscle growth)
 Titin (muscle assembly, structure, elasticity)
• Protein profile differences within Duroc: leanest vs. fattest
TÍTOL DEL
POWER POINT.
PRESENTACIÓ
WP3
GENETIC REGULATION OF FAT
DEPOSITION AND COMPOSITION
• Fat metabolism
 Acetyl-CoA carboxylase (ACACA)
 ATP citrate lyase (ACL)
 Fatty acid synthase (FASN)
 Stearoyl-CoA-desaturase (SCD)
 ∆5 and ∆6 desaturases
• Lipogenic enzymes in 2D and 3D cell culture
• Gene networks: PPAR/RXR transcription factors
 Adipocite differentiation, lipogenesis
TÍTOL DEL
POWER POINT.
PRESENTACIÓ
WP4
ANIMAL WELFARE:
STRESS & MEAT QUALITY
• Pre-slaughtering stress
Best non-invasive measure of stress?
• Genetics of stress tolerance
Gene expression profile differences between
stressed and non-stressed pigs
TÍTOL DEL
POWER POINT.
PRESENTACIÓ
WP5
NEW TECHNOLOGIES FOR
MEASURING PORK QUALITY
• Current methods are slow, expensive, and difficult to
implement on-line
 Fat composition by gas/liquid chromatography
• Future methods should be fast, cheap, and easy to
implement on-line
 Measuring intramuscular and back fat:
• Magnetic immunosensors
• Impedance spectroscopy
• Hormones and stress (cortisol)
TÍTOL DEL
POWER POINT.
PRESENTACIÓ
PORK QUALITY BIOSENSORS WORKSHOP
Lleida 25 October 2012
• From 10 am to 6 pm (2 hours break)
• Oral (15+5 min.) or posters
• 18 presentations in 6 hours (3 pres./hr.)
• Abstract Format
(www.bdporc.irta.es/excelmeat/Workshop.html)
• Audience: EXCELMEAT members, IRTA colleagues,
guest researchers, SMEs, Large Companies, Vet
College...
TÍTOL DEL
POWER POINT.
PRESENTACIÓ
FUTURE PROJECT
• Objective
What can generate income to SMEs?
• Focus
Is GxE the way forward?
• Funding
EU funding research for SMEs
National CDTIs