Fruit Fly Lab Report Basics

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Transcript Fruit Fly Lab Report Basics

Fruit Fly Lab Report
Basics
• Completed individually – everyone’s should be
different except for combined data
• Typed
• Due 1/20 or Earlier – on paper, unless “printer
broken” then email
• 40 points
Format
• Top:
Amy Fansler
Other group members
6D Flies
1/20/2011
4th Period
PHH
The Outcome of Crossing Vestigial Wings with Wild
Type Flies
DO. NOT.
• PLAGIARIZE! This means COPYING and
PASTING without citing!!!!
• I WILL BE ABLE TO TELL
• I WILL GOOGLE SEARCH YOUR SENTENCE
• YOU WILL BE PENALIZED.
Introduction
• Describe Drosophila
• Why we use it
• The traits you are studying (wild type, sepia,
white, vestigial, etc.)
• List possible genotypes and phenotypes of
flies from parental flies
• Exceeds expectations…
Intro, Continued
• Research Question = what are you trying to
find out???
• Example: “If two parent flies are heterozygous
for Vestigial Wings, should their offspring
conform to a 3:1 ratio??”
– Ww x Ww = 3: W- and 1: ww
• What traits are you studying?
Intro, continued
• Hypothesis
– What proportion do you think the offspring should
come out to?
– This should be the “expected” proportion
– Ex: 3:1, or 9:3:3:1 for dihybrid
Intro, continued
• Predictions
– Show punnett squares of your P, F1, and F2 flies
– Summarize expected proportions/percentages
– This explains your hypothesis
• Questions so far???
Materials
• What equipment did you use?
• What was the purpose of that equipment?
• List in bullets – not paragraph form
Methods
• Same as procedure
• What steps did you take to do this experiment
each day?
Results
• Table format
Example Data:
F1 Flies: 16 Wild Type Wings, 0 Vestigial Wings
F2 Flies: 39 Wild Type Wings, 4 Vestigial Wings
F1
F2
Wild Type Wings
16
39
Vestigial Wings
0
4
Total Flies
16
43
Proportion or
Percentage
100% Wild Type
90.6% Wild Type,
9.3% Vestigial
*Remember,
our expected
outcome was
3:1 or 75% to
25%
Chi Square Analysis
• We will do this later once everyone has their
data
• It is to see how reliable your data is… or how
much error there was in your experiment…
Conclusion
• Dominant/Recessive?
• Autosomal/Sex-Linked?
• Why do you say this?? Cite your data
– “Since there were no vestigial wings in the F1
generation, but there were in the F2 generation,
this leads us to believe that vestigial wings are
recessive.” Yadda yadda.
• You will “accept” or “reject” your hypothesis
based on the chi square analysis we do later
• Did it follow the expected outcome?
Resources
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ANYTHING you use from the internet
Citationmachine.net gives you citation
MLA format
Number them, and use numbers during
introduction/conclusion.
Grading
• Final CUMULATIVE exam = 60 points
• Lab report = 40 points
• -5 points for each day late
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Introduction = 15 points
Materials and Methods = 5 points
Results = 10 points
Conclusion = 5 points
Resources = 5 points