Transcript Media:ATPsynthase

ATP Synthase: Subunit Gamma
Lauren Ivey
• Resveratrol binds to gamma, alpha, beta
– Hydrolysis and synthesis
• Hydrophobic pocket between beta and
gamma subunit
• Gamma
– Because beta most likely more highly conserved
– Alpha, beta in larger scale project
ATP synthase animation
Grape to blueberry
Blueberry hits from grape
• TAGTCCGAAACCGGATGAAGAGTGTGAAGAATATC
CAGAAAATTACTAAGGCCATGAAAA
• TGGTTGCAGCATCTAAGCTGCGAGCTATTCAAGTTA
GGGCTGAGAATTCCCGTGGCCTAT
• GGCAGCCATTTACTGCACTTCTTGG
• GTTCTGTTCAATGCTGTCTTGGAGAATGCTACCAGT
GAGCAAGGAGCAAGGATGTCTGCC
• ATGGATAGCTCCAGCAGAAATGCTGGAGACATGCT
TGACCGCCTCACGCTTACTTATAAC
Blueberry to bovine
Grape to Bovine
Blueberry to human
Next Steps
• Amino acid sequences
– Bovine, grape, blueberry
• Beta and alpha
• Interactions with resveratrol structure
• Hypothesis: No hydrophobic pocket and
resveratrol does not bind to ATP synthase in
blueberry and grape
Why do we care?
• cardiovascular disease and neurological
disorders
• cardiac ischemia– Prevents hydrolysis of ATP
• apoptosis
• Selective induction
• Normal cells change binding sites
Gledhill, J.R et al. Mechanism of inhibition of bovine F1-ATPase by resveratrol and related polyphenols. PNAS. 2007; 104(34): 13632-13637.