Transcript Post-column Derivatization - Pickering Laboratories, Inc.

Pickering Laboratories
Corporation
Post-Column
Instruments and Chemistry
Guaranteed Chemistry
• Only Pickering Laboratories Makes the
Chemistry, Columns and Systems
specifically optimized for each analytical
Method.
• Guaranteed to produce excellent
Chromatography:
– Reproducible, Sensitive and Specific
Post-column Derivatization
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Renders visible certain compounds that are normally invisible
by performing a chemical reaction on the substances that
gives them an easily detectable physical property
– Reaction typically produces a strong color or fluorescent product
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Used to increase sensitivity and selectivity in HPLC analysis
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Mixes the stream of effluent flowing from the HPLC column
with a stream of reagent solution
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Compounds and reagent flow into reaction chamber
– Typically heated
– Some methods require two (or even three) reactions in series
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Derivatized compounds pass into a detector
Post-Column Analysis Methods
(Partial List)
• Aminoglycosides
Antibiotics
• Amino Acids
• Biogenic Amines
• Bromate
• Carbamate Pesticides
• Chromium VI
• Formaldehyde
• Glyphosate Herbicide
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Guanidinos
Hexosamines
Mycotoxins
Paralytic Shellfish Toxins
Paraquat & Diquat
Polyether Antibiotics
Sulfa Drugs
Transition/Rare Earth
Metals
US – EPA
(Environmental Protection Agency)
Regulations Specify Pickering Laboratory
• Drinking water regulation 531.2 testing for
Carbamates Specify Pickering chemistry and methods
• Water regulation 547 testing for Glyphosate Specify
Pickering chemistry and methods
AOAC international
(Association of Analytical Communities)
Official Validation
• AOAC Official Method 985.23 – N-Methylcarbamates
Insecticide and Metabolite Residues
• AOAC Official Method 991.08 – Glyphosate and
Aminomethylphosphonic Acid (AMPA) in
Environmental Water
• AOAC Official Method 994.12 – Amino Acids in Feeds
PCX5200 Post-Column
Derivatization Instrument
• Rugged
• Reliable
• Optional PC
Software
• Column safeguards
• Low-pulsation
• Reactor Temp to
130º C
Post-Column Reagents
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Optimized for Post-Column Methods
Chromatographic Grade™
99.9% Purity
Consistency
Pre-mixed for laboratory efficiency
Columns and Guards
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Optimized for Post-Column Methods
Quality Assurance Individually Tested
Reliability
Longevity
Laboratory Cost effectiveness
Pickering Labs.Com
Company
• 23 Years of business
• Direct Sales in US and through 87
international distributors
• Headquarters in Mountain View
California, USA