Transcript (Morus indica L. cv. Anantha) leaves in streptozotocin

Antioxidant role of
mulberry (Morus indica
L. cv. Anantha)
leaves in streptozotocindiabetic rats
a
• Bondada Andallu *,
N.Ch. Varadacharyulub
• a Department of Home Science,
Sri Sathya Sai Institute of
Higher Learning, Anantapur515001, India
• b Department of Biochemistry,
Sri Krishnadevaraya University,
India
系別：生科四甲
學號：91390391
姓名：吳佳煒
Received 20 December 2002 received in revised form 7 July 2003; accepted 7 July
2003
Abstract
•
The antihyperglycemic and
antioxidant role of mulberry
(Morus indica L.) leaves were
investigated.
Model：Wistar rats
I.
II.
Standard diet
25% dry mulberry leaf powder and
standard diet
For 8 weeks
Abstract
♠Key point：
antihyperglycemic and antioxidant
role of mulberry effect：
• on blood glucose
• lipid peroxidation
• reduced glutathione (GSH)
concentrations and on the activity of
glucose-6-phosphate dehydrogenase
(G6PDH)
• various antioxidant enzymes in
erythrocytes
Results：
Abstract
 lipid peroxidation
 fasting blood glucose concentrations
 glutathione GSH concentrations
 activity of glucose-6-phosphate
dehydrogenase (G6PDH)
 antioxidant enzymes
Introduction
• Hyperglycemia 血糖過高症
• Free radicals result in the
consumption of antioxidant
defenses may lead to：
a. disruption of cellular functions
b. oxidative damage to membranes
c. enhance susceptibility to lipid
peroxidation.
Introduction
• Total antioxidant status (TAS) of
serum is reduced in type I diabetes
• The reactive oxygen species are
modulated by antioxidant enzymes：
Glutathione reductase (GR)
glutathione peroxidase (GPx)
glutathione-S-transferase (GST)
superoxide dismutase(SOD)
catalase (CAT)
non-enzymatic scavengers like reduced
glutathione.
Introduction
• Mulberry (M. alba L.) leaves containing many
nutritional components：
•
•
•
•
•
•
•
Flavones
黃酮
Steroids
類固醇
Triterpenes
三烯
amino acids
氨基酸
Vitamins
維生素
Moracin
桑辛素
other trace minerals 無機物
Materials
Animals
• Male rats of Wistar strain from
Hyderabad
weight range 150~200g
air-conditioned room (25° ±1℃)
light from 7 a.m. to 7 p.m.
Divide into 4 groups
Materials
Wistar rats
I： normal control
II：normal treated with
mulberry leaf powder
III：diabetic control
IV：diabetic treated with
mulberry leaf powder
Materials
Leaves
• The mulberry leaves (M. indica L. cv.
Anantha)
• The 4th and the 5th leaves from the
apex of healthy plants were plucked
• washed thoroughly under running
tap water
• shade dried for 3 days and ground to
a fine powder in an electric mixer
Methods
 Groups III and IV (diabetic)
 by a single i.p. injection of streptozotocin
(STZ) (55 mg/kg) prepared in 1 ml of
freshly prepared 0.1 mol/l citrate buffer
after an overnight fast.
 Groups I and II (Control) were injected
with citrate buffer alone.
 Streptozotocin injected animals were
given 5% glucose water for 24 h following
streptozotocin injection to prevent initial
drug-induced hypoglycemic mortality
Methods
streptozotocin
•注射streptozotocin，以破壞分泌胰
島素的β胰島細胞，進而形成高血糖的
情形 。
•增加大鼠之進食量、飲水量及排泄量，
並使體重變輕、血糖升高。
•人類糖尿病的症狀：吃多、喝多、尿多
Methods - Biochemical estimations
• Erythrocytes were separated from
whole blood
• Erythrocytes were washed thrice
with 0.9% NaCl
• suspended in 1 volume of 0.9%
NaCl
• The packed cell volume was
adjusted to 5% with PBS (pH 7.4)
Student's T test
X
ts
n
Results & Discussion
Fasting Blood
Glucose
Fasting blood glucose
decreased food consumption insignificantly
increased the body weight in diabetic rats
• Mulberry
decreased food consumption
insignificantly
increased the body weight in
diabetic rats
fagomine which potentiates the
glucose induced insulin release
similar to the action of
glibenclamide a sulfonylurea drug
which triggers the release of
endogenous insulin
increase in tissue uptake of glucose
by mulberry leaves
桑葉生物鹼fagomine可顯著增加葡萄糖引起的胰島素含量增加，從而降低糖尿病小鼠血
糖。
The effect of mulberry in controlling
hyperglycemia and glycosuria could
be due to:
the N-containing sugars isolated
form mulberry leaves which inhibit
the functions of
α-glucosidase
α-mannosidase
β-galactosidase
diabetic control rats vs. normal controls
glucose-6-phosphate
dehydrogenase
glutathione reductase
glutathione peroxidase
glutathione-S-transferase
superoxide dismutase
Catalase
p < 0.01
13%
34%
20%
30%
46%
59%
CATALASE可氧化分解身體內過量的尿酸 . 將H2O2還原成H2O的反應
Glutathione peroxidase:催化以glutathione為還原劑，將H2O2還原成H2O的反
Diabetic control v.s mulberry
• The activity of glucose-6-phosphate
dehydrogenase (69%; p < 0.01)
• glutathione reductase (95%; p < 0.01),
glutathione peroxidase (151%; p < 0.01)
• glutathione-S-transferase (24%; p < 0.01)
• superoxide dismutase(106%; p < 0.01)
• activity of Catalase 33%
all p < 0.01
Conclusion
• Mulberry leaves are palatable and
effective in controlling
hyperglycemia and glycosuria in
STZ-diabetic rats.
• The leaves exerted a rapid
protective effect against lipid
peroxidation by scavenging O2 and
elevating the activities of
antioxidant enzymes by virtue of
antioxidant flavonoids and moracins
present in the leaves.
桑辛素Moracin 這類化合物顯示了良好的抗菌和抗真菌作用，是植物抗真菌抗生素。
類黃酮 flavonoid 一類不含氮的生物色素，包括花色素&FAA1;和花黃色素。類黃酮在植物中存
在普遍，但在動物中出現較少，具有抗癌的作用。
Question & Answer
Thanks your listening