Transport Across Membranes
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Transcript Transport Across Membranes
You must have lab ready for tomorrow
Lecture #6
Take
in nutrients
Expel waste
Communicate with it’s
environment
Communicate with
neighbouring cells
The plasma membrane must be highly
selective
It must be able to take in a very large
food molecule while preventing very
small and valuable molecules from
leaving the cell
It must recognize and block harmful
foreign substances while expelling the
cell’s toxic waste products
Membranes
within the cell must
also be crossed by important
materials
Ex. in the mitochondria and
chloroplasts reactions occur
which require reactants from
outside the organelle and produce
products that need to leave
The movement of a substance across a
membrane without the need use energy
Diffusion is the main type of passive
transport
Diffusion is the movement of molecules from
a place of higher concentration to a place of
lower concentration
The rate of diffusion depends on the
concentration difference (aka concentration
gradient) between the two areas
1.
2.
Simple Diffusion: the ability of
substances to move across a
membrane unassisted (ex. Water,
Oxygen and Carbon Dioxide)
Facilitated Diffusion: when
substances require help by transport
proteins to cross a membrane (ex.
Sugars or amino acids), though this is
still based on concentration gradient
1. Channel Proteins: a hydrophilic pathway in
a membrane that enables water and ions
(ex. sodium, potassium, calcium and
chloride) to pass through
2. Carrier Proteins: bind to a specific solute
(ex. glucose molecule or particular amino
acid) and transports it by changing shape to
move it across the lipid bilayer. *Each
protein is VERY specific (one for glucose
could not transport fructose) which means
SUPER tight control
Diffusion of water across a membrane
In living cells this movement can
cause swelling and shrinking
depending on the cell’s surrounding
conditions
There are three kinds of surrounding
conditions (hypotonic, hypertonic and
isotonic) and each impacts the cell in a
different way
1.
2.
3.
Hypotonic: a solution that has a lower
solute concentration than another
(water moves into the cell)
Isotonic: a solution that has the same
solute concentration than another
(the cell remains unchanged)
Hypertonic: a solution that has a
higher solute concentration than
another (water moves out of the cell)
The movement of substances across
membranes against their
concentration gradient using pumps
Energy-dependent (about 25% of a
cell’s energy requirements are used
for active transport)
Classified as either primary or
secondary active transport
Use transport pumps
ONLY move specific positively charged
ions (ex. hydrogen)
A hydrogen pump (aka proton pump)in
the plasma membrane pushes hydrogen
ions form the cytosol to the cell exterior
The pump will bind to a phosphate
group from ATP to provide the energy to
move the ion
Uses the concentration gradient of an ion as
it’s energy source
Facilitated by two mechanisms – symport
and antiport
Symport: a solute moves through the
membrane channel in the same direction as
the driving ion
Antiport: the driving ion moves through the
membrane channel in one direction,
providing energy for the transport of
another molecule in the opposite direction