Transcript Welcome to the continuation of Biol 213 Genetics!

Welcome to the continuation
of Biol 213 Genetics!
Genetics News
What’s in the mail?
• EditBase - DNA processing program
• Mystery sequence
Problem Set 3 now available
Lab 2 - long
Feedback welcome
Upcoming events
Monday, Sept 18: Problem day (Problem Set 3)
Friday, Sept 22: Problem day (Problem Set 4)
Monday, Sept 25: First exam (7:00 PM)
Monday, Sept 25: in class….?
Today’s events
DNA Replication
Integration of leading/lagging strand synthesis
Inborn errors of metabolism
How does alkaptonuria work
Beadle & Tatum’s experiment with Neurospora
How does alkaptonuria work
DNA Replication
How can DNA polymerase go left to right
and right to left simultaneously?
Relationship between genes and proteins
One gene - many protein
gene
RNA
Protein
precursor
Relationship between genes and proteins
One gene - many protein
Protein
precursor
lactose
Lactose degrading enzyme
maltose
Maltose degrading enzyme
Relationship between genes and proteins
Many genes - many protein
Relationship between genes and proteins
Many genes - many protein
One gene - One protein
Phenylalanine degradation
Dietary protein
phenylalanine other amino acids
tyrosine
p-hydroxyphenylpyruvic acid
homogentisic acid
Homogentisic
acid oxidase
maleylacetoacetic acid
Phenylalanine degradation
Dietary protein
phenylalanine other amino acids
tyrosine
p-hydroxyphenylpyruvic acid
homogentisicacid
acid
Homogentisic
Homogentisic
acid oxidase
maleylacetoacetic acid
Black oxidation
product
Alkaptonuria
Problems
Phenylalanine degradation
Dietary protein
phenylalanine other amino acids
Phenylalanine
hydroxylase
tyrosine
p-hydroxyphenylpyruvic acid
homogentisic acid
maleylacetoacetic acid
Phenylketones
(neurotoxins)
Life cycle
of yeast
How to get mutants in Neurospora
Haploid Neurospora
radiation
Diploid Neurospora
radiation
screen 1000’s
Auxotroph?
No auxotrophs
How to get mutants in Neurospora
self cross
1000’s
auxotroph?
Could you do this with humans?
back cross
1000’s
self cross
mixed mating types
Analysis of Beadle & Tatum Expt
1. One gene - many enzymes
2. Many genes - one or more enzymes
3. One gene - one enzyme