Transcript AssignTracker - Biology Learning Center

Announcements:
• I got caught checking my cell phone, so in order
to avoid being labelled a hypocrite, you can text
in class, just give me your attention when I
lecture!
• No one wants to go to office hours on a Friday
afternoon  should I change my hours?
• Quiz 3 average: 83%
• Send me emails if you want help on anything but
can’t attend office hours, if you’re still having
trouble with the first weeks material, it isn’t going
to get easier…
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How?
Why?
From strings to
blobs
From there to here
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Week 3: How DNA can ‘mean’
anything; how it can pass that
meaning on (replication)
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Week 4: How DNA can send out a
‘message’ (transcription); how that
‘message’ can be ‘translated’ into
amino acids
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Now: How a string of amino acids is
formed into a functional shape
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What matters
today?
How do we go from the ‘ticker tape’ of an mRNA to a machine?
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machine = 3D object that does stuff
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Who shapes proteins into their shapes? Terminology: ‘folding’
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How specific amino acids and protein shapes give rise to
operational machines that perform body tasks
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Meet the building
blocks
• There are only about 5 ways molecular
surfaces can be
• What are they?
• Amino acid easter egg hunt--find the one(s)
that...
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How does it feel?
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http://upload.wikimedia.org/wikipedia/commons/a/a9/Amino_Acids.svg
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Ooey gooey rich & chewy inside...
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Protein folding, oil not mixing with water, and
membrane formation all reflect the same
principle
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In protein folding, the constraint is that the
individual units are all attached to a pair of
neighbors
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Many proteins need no further ‘instruction’ than
their sequence & water to correctly assume their
superhero identity
If THIS is true…
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Pencils made of….
http://www.nanoenhanced-wholesaletechnologies.com/faq/carbo
n-forms.htm
If THIS is true…
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Pencils made of….
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Paper made of…
Question Authority
To your pencils, index cards & oil-water mixes!
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Color one face of an index card with pencil
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Cut out circles with hole puncher
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Predict: what will happen?
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Drop into oil/water bottles; mix
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Your turn
You ‘fold’ a protein: ProFolder (Bio181L_Go)
Show me each solution (Q. 3)
Leave the 2nd one on screen
Profolder features
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Destinations (lower right) => Folding
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Top: amino acid string
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Squares: places amino acids could go. Note ‘Undo last’ button
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Two spots--use one to improve upon what you did in the other
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Bottom: note that when you mouseOver an amino acid, it’s
structure & ‘feel’ are shown
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How does yours compare?
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Other ‘rules’ of folding
(+)
(-)
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Images of 3D
Q. 4
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How?
Life’s blood:
Hemoglobin
Why?
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Hemoglobin: overview
http://www.youtube.com/watch?v=WXOBJEXxNEo&feature=related
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Scale & role
Small capillary
(blood vessel)
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Tetramer:
4 protein chains
(~145 amino acids)
Heme:
C34H32O4N4Fe
Oxygen molecule
2 atoms
Image source: http://www.myoptumhealth.com/portal/ADAM/item/Sickle+cell+disease
1-2-3-4 protein!
http://compbio.pbworks.com/w/page/16252897/Introduction-and-Basic-Molecular-Biology
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Hemoglobin: what is
it?
Image source:
http://themedicalbiochemistrypage.org/hemoglobin-myoglobin.html
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What you’ll learn today
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How hemoglobin’s amino acid sequence
generates its structure
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Why hemoglobin is a tetramer (gang of four)
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Todays assignments
30% Worksheet: easter egg hunt
60% points hemoglobin tutorial
10% points hemoglobin mini-research
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Adam
Alice
Anders
Jillian
Montez
Karl
Blake
Hemoglobin_intro
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Turn OFF Wi-Fi; QUIT safari (not close window)
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Read...
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the instructions on each question...
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the instructions on the webpage...
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all the words of each question...
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Ask yourself: will you be the monkeys at the
typewriter, or Shakespeare?
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CLOSE EACH PAGE WHEN YOU’RE DONE
WITH IT!
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On the relative likelihood
of accidents...
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What specifically would it take
for...
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A lysine to become a glycine?
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To your codon tables! (page 4-8 in lab manual)
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How often is that going to happen?
More on your
disease!
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Get into your Genetic Disease groups
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Write up Part 2 of the assignment (view link on course homepage)
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FOLLOW THE RUBRIC
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Do you remember what disease you have? You should’ve written
it down… If not, ask me.
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EMAIL ME YOUR ANSWERS: [email protected]
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Provide your Genetic Disease Name in Subject
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Homework