Transcript SURVEY OF BIOCHEMISTRY

SURVEY OF BIOCHEMISTRY
Course Review
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PRS-1
• Catalytic efficiency is given by what
expression?
1. kcat
2. kcat/KM
3. Vmax/KM
4. [E]T x kcat
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PRS-1
• Catalytic efficiency is given by what
expression?
1. kcat
2. kcat/KM
3. Vmax/KM
4. [E]T x kcat
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PRS-2
• Which amino acid is an alpha-helix
breaker?
1.
2.
3.
4.
Y
W
V
P
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PRS-2
• Which amino acid is an alpha-helix
breaker?
1.
2.
3.
4.
Y
W
V
P
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PRS-3
• Amino acids are linked by what types
of bonds in proteins?
1.
2.
3.
4.
Disulfide bonds
Peptide bonds
Both #1 and #2
None of the above
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PRS-3
• Amino acids are linked by what types
of bonds in proteins?
1.
2.
3.
4.
Disulfide bonds
Peptide bonds
Both #1 and #2
None of the above
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PRS-4
• Which cofactors are required for
the aldolase reaction?
1. NAD+
2. CoA
3. FAD
4. None
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PRS-4
• Which cofactors are required for
the aldolase reaction?
1. NAD+
2. CoA
3. FAD
4. None
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PRS-5
• Which of the following is NOT an
example of a cofactor?
1.
2.
3.
4.
Glucose
Thiamine Pyrophosphate
Pyridoxal Phosphate
Coenzyme A
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PRS-5
• Which of the following is NOT an
example of a cofactor?
1.
2.
3.
4.
Glucose
Thiamine Pyrophosphate
Pyridoxal Phosphate
Coenzyme A
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PRS-6
• What function does glucagon exhibit?
1. Increasing glycogen breakdown
2. Binding to receptors on liver cells
3. Triggering increased levels of cAMP
production
4. All of the above
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PRS-6
• What function does glucagon exhibit?
1. Increasing glycogen breakdown
2. Binding to receptors on liver cells
3. Triggering increased levels of cAMP
production
4. All of the above
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PRS-7
• Pyruvate carboxylase converts ___ to
_____.
1. Acetyl CoA to pyruvate
2. Pyruvate to acetyl Coa
3. Pyruvate to Oxaloacetate
4. Pyruvate to Phosphoenolpyruate
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PRS-7
• Pyruvate carboxylase converts ___ to
_____.
1. Acetyl CoA to pyruvate
2. Pyruvate to acetyl Coa
3. Pyruvate to Oxaloacetate
4. Pyruvate to Phosphoenolpyruate
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PRS-8
• A point mutation in which a purine is
replaced by a pyrimidine is called:
1.
2.
3.
4.
Transversion
Transition
Insertion
Deletion
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PRS-8
• A point mutation in which a purine is
replaced by a pyrimidine is called:
1.
2.
3.
4.
Transversion
Transition
Insertion
Deletion
See Section 25-4
From Reading not mentioned in
lecture…
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PRS-9
• Which statement is true?
1. Hemoglobin binding of CO2 stabilizes the T
form
2. The sickle cell form of hemoglobin contains
a point mutation (E->V)
3. 2,3-Bisphosphoglycerate decreases
hemoglobin’s affinity for O2
4. All of the above
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PRS-9
• Which statement is true?
1. Hemoglobin binding of CO2 stabilizes the T
form
2. The sickle cell form of hemoglobin contains
a point mutation (E->V)
3. 2,3-Bisphosphoglycerate decreases
hemoglobin’s affinity for O2
4. All of the above
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PRS-10
• Which of the following is a unit of
O2 pressure?
1.
2.
3.
4.
mm Hg
mol/L
%
nm
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PRS-10
• Which of the following is a unit of
O2 pressure?
1.
2.
3.
4.
mm Hg
mol/L
%
nm
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PRS-11
• See Figure 1.
The shift from blue to red indicates:
1. An increase in pH
2. A decrease in pH
3. An increase in hemoglobin concentration
4. A decrease in hemoglobin concentration
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PRS-11
• See Figure 1.
The shift from blue to red indicates:
1. An increase in pH
2. A decrease in pH
3. An increase in hemoglobin concentration
4. A decrease in hemoglobin concentration
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PRS-12
• Which are 5-C monosaccharides?
1. Glucose and ribose
2. Ribose and fructose
3. Ribose and mannose
4. None of the combinations above
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PRS-12
• Which are 5-C monosaccharides?
1. Glucose and ribose
2. Ribose and fructose
3. Ribose and mannose
4. None of the combinations above
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PRS-13
• Which reaction is an example of an
acid-base catalyzed reaction?
1.
2.
3.
4.
Hexokinase
Pyruvate kinase
Phosphoglucose isomerase
Pyruvate dehydrogenase
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PRS-13
• Which reaction is an example of an
acid-base catalyzed reaction?
1.
2.
3.
4.
Hexokinase
Nucleophilic attack
Pyruvate kinase Nucleophilic attack
Phosphoglucose isomerase
Pyruvate dehydrogenase
5-step Reaction
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PRS-14
• Which of the following is not a step in
the pyruvate dehydrogenase
mechanism?
1. Decarboxylation
2. Reduction of FAD to FADH2
3. Oxidation of NADH to NAD+
4. Production of acetyl-CoA
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PRS-14
• Which of the following is not a step in
the pyruvate dehydrogenase
mechanism?
1. Decarboxylation
2. Reduction of FAD to FADH2
3. Oxidation of NADH to NAD+
4. Production of acetyl-CoA
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Pyruvate Dehydrogenase Rxn
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PRS-15
• Which of the following is not a
disulfide reducing agent?
1.
2.
3.
4.
Cysteine
Beta-mercaptoethanol
Lipoic acid
Linoleic acid
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PRS-15
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PRS-16
• cDNA _____.
1.
Is DNA found in the cytosol of a cell.
2.
Comes from reverse transcription of mRNA.
3.
Stands for “capped” DNA.
4.
Contains the DNA sequence from the entire
genome.
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PRS-16
• cDNA _____.
1.
Is DNA found in the cytosol of a cell.
2. Comes from reverse transcription of mRNA.
3.
Stands for “capped” DNA.
4.
Contains the DNA sequence from the entire
genome.
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PRS-17
• Which are nonpolar amino acids?
1. Gly, Val, Lys
2. Arg, Leu, Met
3. Ile, Pro, Phe
4. Ala, Met, Ser
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PRS-17
• Which are nonpolar amino acids?
1. Gly, Val, Lys
2. Arg, Leu, Met
3. Ile, Pro, Phe
4. Ala, Met, Ser
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PRS-18
• Beta sheets can contain _____.
1. Antiparallel strands
2. Parallel strands
3. Interstrand H-bonds
4. All of the above.
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PRS-18
• Beta sheets can contain _____.
1. Antiparallel strands
2. Parallel strands
3. Interstrand H-bonds
4. All of the above.
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PRS-19
• The hydrophobic effect is driven by
______.
1.
2.
3.
4.
Enthalpy
Entropy
The concentration of water
Temperature
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PRS-19
• The hydrophobic effect is driven by
______.
1.
2.
3.
4.
Enthalpy
Entropy
The concentration of water
Temperature
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PRS-20
• Which is a major component of brain
lipids?
1.
2.
3.
4.
Fatty acids
Triglycerides
Gangliosides
Phospholipids
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PRS-20
• Which is a major component of brain
lipids?
1.
2.
3.
4.
Fatty acids
Triglycerides
Gangliosides
Phospholipids
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Reminders
• Final exam is closed-book.
• Bring a calculator.
• Study by:
– Reading the text
– Reviewing suggested HW problems
– Reviewing What You Should Know
summaries (no more added)
– Reviewing past exams
– Studying lecture notes
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CETL Course Survey(s)
• Course-Instructor Opinion Survey (CIOS)
– http://www.coursesurvey.gatech.edu
– Also on T-Square Home Page
• Pilot Survey
– Go to T-Square: Announcements for link to
survey
– Focus groups to learn more about student
opinions
– Deadline: August 3, 2008
– IRB Approval requested: minimal risk
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