Transcript Updates from MetaSUB Beijing

Updates from MetaSUB Beijing
Chu Ping
Beijing Children’s Hospital
Beijing Key Laboratory for Pediatric Diseases of
Otolaryngology, Head and Neck Surgery
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Content
Brief Introduction of Beijing
Subway
Pilot Study
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Brief Introduction of
Beijing Subway
• The Beijing Subway has 18 lines, 334 stations and 554 km of track in
operation, and is the second longest subway system in the world after
the Shanghai Metro.
• The subway is the world's busiest in annual ridership, with 3.41
billion trips delivered in 2014, averaging 9.2786 million per day, with
peak single-day ridership reaching 11.5595 million.
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Lines
• Beijing Subway lines generally follow the checkerboard layout of the
city.
• Most lines through the urban core (outlined by the Line 10 loop) run
parallel or perpendicular to each other and intersect at right angles.
• Most lines through the urban core run parallel or perpendicular to
each other and intersect at right angles.
• Of the 18 lines, 12 are numbered and the other six are named after
destinations in the outer suburbs.
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History
• 1953–1965: origins
– The Beijing Subway opened in 1969 and is the oldest metro system in mainland
China. The subway has undergone rapid expansion since 2002, as only two lines
were in service before then.
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History
• 1965–1981: the slow beginning
• 1981–2000: two lines for two decades
• 2001–2008: planning for the Olympics
• 2008–present: rapid expansion
– After the Chinese government announced a ¥4 trillion economic stimulus
package in November 2008, the Beijing urban planning commission further
expedited subway building plans, especially for surface light rail to suburban
districts that are cheaper to build.
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Content
Brief Introduction of Beijing
Subway
Pilot Study
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Sampling Position
Yonghegong
Nanlishilu
Dawanglu
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The sampling process
• Sampling point location
– Handrail of elevators
– Bench
• Swab the entire surface for 3 minutes
• 4 replicates per station
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Experimental Design
Sampling
DNA
Extraction
Library
Prep
Data
Mining
DNA Seq
• DNA purification
– MoBio DNA Extraction Kit
• Library preparation
– Nextera XT 96 Kit
• Sequencing
– Illumina X10 Sequencer, 150PE, 60M reads/sample
• Data mining
– Diamond Blasting and Megan LCA Analysis
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Thank you
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