Transcript Supplementary Figure 1 (ppt 246K)

Supplementary Figure 1: Obatoclax-induced cell death is mainly
independent of Bax and Bak
a) MTT Assay
b) Colony Forming Assay
100
wt
DKO
% viability
80
wt
control
DKO
Obatoclax control
0.1 mM
Obatoclax
0.1 mM
60
40
20
0
0.1
0.3
1
3
10
Obatoclax (mM)
Supplementary Figure 1: Obatoclax-induced cell death is mainly independent of Bax and Bak
(a) Wildtype (wt) MEFs or Bax/Bak double knockout (DKO) MEFs were exposed to the indicated concentrations of
obatoclax for 48 h. Viability was assessed by MTT assay. Data are mean + SEM of 4 experiments. (b) Wildtype (wt)
MEFs or Bax/Bak double knockout (DKO) MEFs were seeded at low cell density and exposed to 0.1 mM obatoclax for
48 h. After drug treatment, cells recovered for 96 h to allow colony formation before staining with cesyl-violet.