Transcript Simultaneous Antisolvent Synthesis and Stabilization of

Formation of Drug
Nanoparticles using Solvents,
Polymers and Cellulose.
Measuring Long term stability as
a Function of time
By
Chintal Desai
Dr. Somenath Mitra’s Profile
EDUCATION
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Ph.D Analytical Chemistry, 1988, Southern Illinois University, Carbondale, Illinois. Advisor: Prof. John B. Phillips
M. S. Environmental Engineering, 1984, Southern Illinois University, Carbondale, IL.
B. S. Chemical Engineering, 1981, Indian Institute of Technology, Kharagpur, India.
WORKING AS ACTING CHAIR IN DEPT. OF CHEMISTRY AND ENVIRONMENTAL
SCIENCE, NJIT.
RESEARCH INTEREST
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Sensors and analytical instrumentation; MEMS, lab-on-a-chip, microfluidics; thin-film sensors and devices using
conducting and semiconducting polymers; instrumentation/devices based on membrane separation.
Nanotechnology, carbon nanotube synthesis and functionalization.
RESEARCH SCHOLARS WORKING UNDER HIM
Yuhong Chen, Ornthida Sae-Khow , Chaudery Hussain, Susana Addo Ntim, Xiangxin Meng, Kenneth Gethard, Chintal Desai
TOTAL 7 STUDENTS AND MORE THAN 21 STUDENTS HAVE COMPLETED THEIR Ph.D UNDER HIM
Chintal Desai’s Profile
Eduaction
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Persuing Ph.D Analytical Chemistry, NJIT, Newark, NJ. Advisor: Prof.
Somenath Mitra
M. S. Analytical Chemistry, 2002 , South Gujarat University, India.
B. S. Chemistry, 2000, B. P. Baria Science College, India.
Experience and something about Myself
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Analytical chemist (Themis) India.
Senior Chemist (Atul Pvt. Ltd.) India.
Worked as a Volunteer for AIDS Awareness Program.
Part time Tutor in University and in Community.
Objective
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Antisolvent method is used to make
nanoparticles for hydrophobic drugs.
Main Goal was to use different Solvents,
Polymers and Surfactants to achieve smaller
particle sizes.
Edible films were also made using PEO, PVP
and Tween 80.
Antisolvent Method
Drug Solution
Antisolvent
containing polymers
Precipitation
Nanoparticles
Nanoparticles
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Nanotechnology originates from the Greek word
meaning “dwarf”.
The term “nanotechnology” was first used in 1974,
by Norio Taniguchi
1nm=10-9m, which is tiny, only the length of ten
hydrogen atoms, or about one hundred thousandth
of the width of a hair.
NP has a much grater surface area per unit mass
compared with larger particles, leading to greater
reactivity.
Nanosuspension / Colloids
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In Chemistry, a suspension is a heterogenous fluid
containing solid particles that are sufficiently large for
sedimentation. e.g.(Sand in water)
A colloid is a type of mechanical mixture where one
substance is dispersed evenly throughout another.
(e.g.Milk)
Unlike colloids, suspensions will eventually settle .
Also colloids have smaller particle size compare to
Suspension.
Why We are making Drug nanoparticles
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To improve drug bioavailability
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To enhance dissolution rate for poorly water
soluble drug
Bioavilability and hydrophobicity of drug
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Bioavailability is a pharmacokinetic term that
describes the rate and extent to which the
active drug ingredient is absorbed from a
drug product and becomes available at the
site of drug action.
Bioavailability is concerned with how quickly
and how much of a drug appears in the blood
after a specific dose is administered.
Dissolution Rate
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Dissolution is a standardized method for
measuring the rate of drug release from a
dosage form.
Drug release in the body measured ‘in-vivo’
from plasma or urine concentrations .
Model Drug, Polymer and surfactant
Griseofulvin (GF)
Sodium Dodecyl Sulfate
Hydroxypropyl Methyl Cellulose
Suspensions of GF:
(a) blank containing cellulose and surfactant;
(b) unstable suspension;
(c) stabilized with cellulose and SDS.
Particle Size Distribution
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The particle size distribution (PSD) of a powder, or
granular material, or particles dispersed in fluid, is a
list of values or a mathematical function that defines
the relative amounts of particles present, sorted
according to size. PSD is also known as grain size
distribution.
The method used to determine PSD is called particle
size analysis, and the apparatus a particle size
analyzer.
Particle Size Distribution of
FNB/GF stabilized by HPMC and SDS
6
Volume (%)
5
4
3
2
1
0
0
2
4
6
8
10
12
Particle Diameter (um)
(a) FNB stabilized by HPMC and SDS;
(b) GF stabilized by HPMC and SDS.
SEM
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The scanning electron microscope (SEM)
is a type of electron microscope that images
the sample surface by scanning it with a
high-energy beam of electrons.
The electrons interact with the atoms that
make up the sample producing signals that
contain information about the sample's
surface topography, composition and other
properties such as electrical conductivity.
SEM images of GF particles
GF stabilized by HPMC and SDS
SEM images of GF- loaded polymer film
Top Surface
Cross section
Mean diameter of drug particles as a function of
time
GF stabilized with HEC and SDS, GF stabilized with HPMC
Mean Diameter as a Function of Time
Mean D iameter as a fu n c tio n o f time
16
14
Mean Diameter(um)
12
10
A c etone
8
DMS O
6
4
2
Time (hr)
0
0
10
20
30
T im e (Hr)
40
50
60
Edible Film
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Drug-loaded polymer
films were prepared
by solvent
evaporation
technique from
polymer casting
solution.
Results/Conclusions
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Anti-solvent synthesis of nano/micro scale drug particles with simultaneous
stabilization using different solvents, cellulose derivatives and a surfactant
(SDS) is reported.
The mean diameter of the small particles grew with time, while the overall
particle size distribution showed a decrease average particle size due to
sedimentation.
The result showed that small particles size were achieved using DMSO as a
solvent instead of Acetone or VP.
The result also showed that a mixture of cellulose and SDS reduced the
average particle size more effectively than either only cellulose or SDS.
Scanning electron microscopy showed crystalline nature of the particles
formed from this process, and Raman Spectroscopy confirmed the presence
of the drug molecule in these crystals.