Transcript PowerPoint Format - Kinexus Bioinformatics Corporation

Overview of Our Integrated
Proteomics and Bioinformatics
Services Platform
The Systems Proteomics Company
2011 March 25
The Kinexus Discovery Platform
IN VIVO
SERVICES
KINEX
MICROARRAY
SERVICES
IMMUNOHISTOCHEM
GRAPHICS
SERVICES
KINETWORKS
BLOTTING
SERVICES
MASS
SPEC
SERVICES
IN SILICO
SERVICES
PROBE
PRODUCTION
SERVICES
 We have created a highly integrated platform of unique
proteomics and bioinformatics services to identify disease
biomarkers and drug targets and to foster signal transduction
research
 Our proteomics services are unmatched by any company for
convenience, breadth, accuracy, cost and turn-around time
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Services
IN VIVO
SERVICES
CELL CULTURE
KINEX
MICROARRAY
SERVICES
KINETWORKS
BLOTTING
SERVICES
12
16 cell
cell lines
lines
MASS
SPEC
SERVICES
IN SILICO
SERVICES
PROBE
PRODUCTION
SERVICES
Kinexus offers cell
lysates from 16 of the
most commonly
studied human tumour
cell lines prepared
according to client
specifications for
proteomics analysis
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Services
IN VIVO
SERVICES
CELL CULTURE
ANIMAL TISSUES
KINEX
MICROARRAY
SERVICES
KINETWORKS
BLOTTING
SERVICES
12
16 cell
cell lines
lines
>40 monkey, rat
+ mouse tissues
MASS
SPEC
SERVICES
ANTIBODY
VALIDATION
PROTEIN
CHARACTERIZATION
IN SILICO
SERVICES
PROBE
PRODUCTION
SERVICES
Clients can also
choose from over 230
pre-made cell and
tissue lysates for use
in our proteomics
services
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Microarray Services
IN VIVO
SERVICES
KINEX
MICROARRAY
SERVICES
ANTIBODY
MICROARRAYS
KINETWORKS
BLOTTING
SERVICES
MASS
SPEC
SERVICES
IN SILICO
SERVICES
PROBE
PRODUCTION
SERVICES
>800
>650 antibodies
Close up of 1 of 16 grids on a Kinex™
Antibody Microarray - control (blue) and
treated (red) have been overlaid and
staggered for comparison.
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Microarray Services
IN VIVO
SERVICES
KINEX
MICROARRAY
SERVICES
KINETWORKS
BLOTTING
SERVICES
MASS
SPEC
SERVICES
ANTIBODY
MICROARRAYS
>800
>650 antibodies
REVERSE
MICROARRAYS
>230 lysates
IN SILICO
SERVICES
PROBE
PRODUCTION
SERVICES
Close up of a section of the Standard
Kinex™ Reverse Lysate Microarray. Custom
KRLM chips can also be produced for our
clients with their own cell/tissue lysates.
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Microarray Services
IN VIVO
SERVICES
KINEX
MICROARRAY
SERVICES
KINETWORKS
BLOTTING
SERVICES
MASS
SPEC
SERVICES
ANTIBODY
MICROARRAYS
>800
>650 antibodies
REVERSE
MICROARRAYS
>230 lysates
PROTEIN KINASE
MICROARRAYS
~200 kinases
IN SILICO
SERVICES
PROBE
PRODUCTION
SERVICES
Close up of a section of the Kinex™ Protein
Kinase Microarray. The binding of an ATP
analogue to active protein kinase is shown.
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Microarray Services
IN VIVO
SERVICES
KINEX
MICROARRAY
SERVICES
KINETWORKS
BLOTTING
SERVICES
MASS
SPEC
SERVICES
ANTIBODY
MICROARRAYS
>800 antibodies
>650
REVERSE
MICROARRAYS
>230 lysates
PROTEIN KINASE
MICROARRAYS
~200 kinases
PEPTIDE
MICROARRAYS
IN SILICO
SERVICES
PROBE
PRODUCTION
SERVICES
Close up of a section of the
Kinex™ Kinase Substrate
Microarray chip. The binding
of a phosphate detection dye
to phosphorylated peptides
is shown.
~450 peptides
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Multi-Immunoblotting Services
IN VIVO
SERVICES
KINEX
MICROARRAY
SERVICES
Kinases
Phosphatases
KINETWORKS
BLOTTING
SERVICES
PAN-SPECIFIC AB
MASS
SPEC
SERVICES
IN SILICO
SERVICES
PROBE
PRODUCTION
SERVICES
2 screens
KPKS-1.2 multi-immunoblot showing
half of the protein kinases detected
with this screen.
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Multi-Immunoblotting Services
IN VIVO
SERVICES
KINEX
MICROARRAY
SERVICES
KINETWORKS
BLOTTING
SERVICES
MASS
SPEC
SERVICES
IN SILICO
SERVICES
Kinases
Phosphatases
PAN-SPECIFIC AB
2 screens
Phosphosites
PHOSPHOSPECIFIC AB
4 screens
PROBE
PRODUCTION
SERVICES
Overlay of two KPSS multi-immunoblots
showing the effects of staurosporin on Jurkat T
cell phosphorylation. Red bands correspond to
increased phosphorylation and blue bands
reveal decreases in phosphorylation.
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Multi-Immunoblotting Services
IN VIVO
SERVICES
KINEX
MICROARRAY
SERVICES
KINETWORKS
BLOTTING
SERVICES
MASS
SPEC
SERVICES
IN SILICO
SERVICES
Kinases
Phosphatases
PAN-SPECIFIC AB
2 screens
Phosphosites
PHOSPHOSPECIFIC AB
4 screens
CUSTOM AB
2 screens


PROBE
PRODUCTION
SERVICES
KCPS 1.0 - 18 antibodies
tested on 1 lysate sample
KCSS 1.0 - 8 cell/tissue
samples tested with up to 3
antibodies
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Mass Spectrometry Services
IN VIVO
SERVICES
KINEX
MICROARRAY
SERVICES
KINETWORKS
BLOTTING
SERVICES
Identify crossreactive proteins
MASS
SPEC
SERVICES
PROBE
PRODUCTION
SERVICES
PROTEIN ID
PROTEOME
PHOSPHORYLATION
Identify phosphosites in a protein
IN SILICO
SERVICES
Track hundreds of
phosphosites
PROTEIN
PHOSPHORYLATION
KINASE SUBSTRATE
PROFILING
Identify kinase
Substrate proteins
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In Silico Prediction Services
IN VIVO
SERVICES
KINEX
MICROARRAY
SERVICES
KINETWORKS
BLOTTING
SERVICES
MASS
SPEC
SERVICES
Obtain a specificity
matrix for a kinase
Identify kinases
for a phosphosite
IN SILICO
SERVICES
PROBE
PRODUCTION
SERVICES
KINASE SPECIFICITY
PREDICTION
KINASE MATCH
PREDICTION
Identify substrates
of a kinase
PHOSPHOPROTEIN
MATCH PREDICTION
Identify kinases for
many phosphosites
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Probe Production Services
IN VIVO
SERVICES
KINEX
MICROARRAY
SERVICES
KINETWORKS
BLOTTING
SERVICES
MASS
SPEC
SERVICES
Active enzymes
and substrates
IN SILICO
SERVICES
PROBE
PRODUCTION
SERVICES
RECOMBINANT
PROTEINS
SYNTHETIC
PEPTIDES
Soluble peptides
Peptides on
macroarrays
PEPTIDE
ARRAYS
Peptides on
microarrays
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Antibody-driven Biomarker Discovery
PREPARATION
In Vivo custom cell culture and treatment
DISCOVERY
Kinex™ Antibody Microarrays
VALIDATION
Kinetworks™ Multi-immunoblotting
VALIDATION
Kinex™ Reverse Lysate Microarrays
LOCATION
IHC Immunohistochemistry
CORRELATION
KiNET and SigNET websites
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Protein Kinase Drugs
 More than 30% of world
EGF receptor 3D structure with kinase
inhibitor drug Tarceva
pharmaceutical research is
focused on protein kinases
 Over 400 human diseases
linked to defects in kinase
signalling
 19 drugs approved and > 150
in clinical trials
 Most kinase inhibitors in clinical
development are for cancer
treatment
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Kinexus Discovery Platform Assists All Phases of
Drug Discovery and Development
DRUG TARGET
DISCOVERY
DRUG TARGET
VALIDATION
PROTEIN
SCREENING
IN VIVO
CELL/TISSUE
SCREENING
MICROARRAYS
MULTI-BLOT
DRUG
DISCOVERY
DRUG
ANALYSIS
HTP COMPOUND
SCREENING
MASS SPEC
CELLS
MECHANISM
IN SILICO
ANIMALS
TOXICOLOGY
HUMAN PHASE
I, II + III TRIALS
PATIENT
SCREENING
PRE-CLINICAL
VALIDATION
CLINICAL
VALIDATION
BIOMARKER
DISCOVERY
PROBE
PRODUCTION
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Kinexus Services for Kinase Drug Discovery
DRUG TARGET
KINASE
DISCOVERY
PROTEIN
SCREENING
KINASE
SUBSTRATE
DISCOVERY
KINASE ASSAY
DEVELOPMENT
CELL/TISSUE
SCREENING
HTP COMPOUND
SCREENING
KINASE DRUG
SPECIFICITY
Kinex™ KAM
In Silico - IKSP
Kinetworks™
Kinex™ - KKSM
In Silico Prediction
Peptide Production
KiNET DB
Kinase Testing - IKPT
SigNET KB
CELLS
MECHANISM
In Silico - IKMP
Kinex™ KAM - CKSP
Mass Spec - MSKS
Binding Assays - Kinex™ - KPKM
Kinex™ KPKM
Activity Assays - KICP
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KiNET DataBase
Online at www.kinet.ca
KiNET
DATABANK
 Kinetworks™ Immunoblotting DataBase
 First launched in 2006
 Features results from >6,000 immunoblots
 Over 200,000 protein measurements
 Search by protein, treatment or cell/tissue
 Over 95% unpublished data
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SigNET KnowledgeBank
Online at www.phosphonet.ca
SigNET
KNOWLEDGEBANK
PhosphoNET KnowledgeBase
Free, online access
>92,000 confirmed and 560,000 predicted
human phosphorylation sites
>23,000 human proteins
Functional information for ~2% of phosphosites
Evolutionary analyses
Kinase prediction
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www.kinexus.ca
ADVISORY BOARD
Kinexus Bioinformatics Corp.
Suite 1, 8755 Ash Street
Vancouver, B.C.
Canada V6P 6T3
Phone: 1-604-323-2547
Facsimile: 1-604-323-2548
E-mail: [email protected]
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