Transcript Slide 1

Neurotransmitter
Release
High
voltage
Low voltage
Mechanism of Exocytosis
synapsins anchor vesicle to cytoskeleton
Ca2+ influx
activates Ca2+/calmodulin dependent kinase (CaMK)
phosporylaton of synapsin I
release of vesicle from cytoskeleton
move to active zone
Rab3A binds to vesicle
hydrolysis of Rab GTP to GDP + PO4
targeted to docking site at active zone
interaction of v-SNARES and t-SNARES
docking and membrane fusion
exocytosis and release of Rab into cytoplasm
t-SNARES = syntaxin and SNAP25
v-SNARE = synaptobrevin (VAMP)
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