Transcript Eigenmannia virescens Transparent Knife Fish

Structural Correlates of Anesthetic
Potency in Alkyl Alcohols
James Sidie PhD
Ursinus College
Eigenmannia virescens
Transparent Knife Fish
WEAKLY ELECTRIC FISH
CONTINUOUS EOD / PULSE EOD
ELECTRIC ORGAN DISCHARGE (EOD)
Eigenmannia virescens
MEDULLARY PACEMAKER NUCLEUS
Eigenmannia virescens
EXPERIMENTAL CONFIGURATION
EFFECT OF CHAIN LENGTH ON EOD
FREQUENCY - 50 ppm
EFFECT OF CHAIN LENGTH ON EOD
FREQUENCY - 25ppm
CUBIC SPLINE ANALYSIS - FIRST
FOUR MINUTES OF EXPOSURE TO
ALCOHOL
EFFECT OF CHAIN LENGTH ON EOD
FREQUENCY - 12.5 ppm
ANESTHETIC POTENCY OF
UNSATURATED OCTANOLS
MOLECULAR MODELLING OF
UNSATURATED OCTANOLS
EFFECT OF TEMPERATURE (25C/30C)
ON OCTANOL EOD DEPRESSION
EFFECT OF PKC INHIBITOR
(CALBIOCHEM Go6983) ON OCTANOL
CONCLUSIONS
• ANESTHETIC POTENCY OF ALKYL ALCOHOLS AT
12.5/25/50 PPM IS POSITIVELY CORRELATED
WITH CHAIN LENGTH UP TO 10 CARBONS
• THE MORE TERMINAL THE POSITION OF THE
-OH GROUP, THE GREATER THE POTENCY OF
THE OCTANOL ISOMER
• THE PRESENCE OF A DOUBLE BOND
DECREASES ANESTHETIC POTENCY AS A
FUNCTION OF BOND POSITION
• PENTADECAFLUOROOCTANOL HAS NO
ANESTHETIC EFFECT
ACKNOWLEDGEMENTS
DR. ERIC WILLIAMSEN DEPT. OF CHEMISTRY
JUDY LYTLE
ANGELO LEPORE
BETH HOLLOWAY
JENNIFER DERUYTER
HHMI
MERCK / AAAS
MOLECULAR MODELLING OF
1,8 OCTANEDIOL
Percent of Normalized Values at
Decanol
TRENDS IN PHYSIOLOGICAL AND
CONCENTRATION DATA
120
100
80
Concntration Data
Physiological Data
60
40
20
0
C6H13OH
C7H15OH
C8H17OH
C9H19OH C10H21OH
GC CONCENTRATION
MEASUREMENTS FOR GOLDFISH
Average Percent of Initial
Concentration
Percent Alcohol Concentration of Initial
Concentrationl for Goldfish
100
80
60
40
20
0
5
6
7
8
9
Num ber of Carbons in Alcohol Chain
10