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Effect of climate on plant growth and level of adaptogenic compounds Project meeting BARENTSHERBS, Rovaniemi, March 2008 Inger Martinussen, Gunnlaug Røthe, Kirsten Jacobsen & Hermod Nilsen • How does climate effect the quality of the herbs? • Quantity and pattern of adaptogenic compounds Test field Serratula coronata, Tromsø, established 2006 • Stratification March-April 2006 • Start germination 18. April • Planting July 2006 • Harvesting July and September 2007 Repeat 1 140.00 Repeat 2 80.00 60.00 40.00 20.00 0.00 Hight No. Dead F.w. (g) D.w. (cm) Leaves leaves tot. (g) TS % D.w. leaves (g) Repeat 3 Field 100.00 Grass 120.00 Ecdysteriods in Serratula from 2006 test field Ecdysteroid (%) 20-hydroxyecdysone JULY 0.42 25S-inokosterone ecdysone 0.15 0.06 •Plants 1 year old •NB! Only 1 sample pr. harvest SEPTEMBER 0.28 6m Serratula Leuzea Rhodiola I I I Test field Serratula, Leuzea and Rhodiola, Tromsø, established 2007 Border 1 m ? Leuzea Rhodiola Serratula II II II 12 m Border 1 m ? Rhodiola Serratula Leuzea III III III Fertilization 40 kg ”Marihøne” organic fertilizer Kg/daa • Size of the field 12 m X 3 m = 36 • 30 cm between plants • 40 cm between rows • 50 plants pr. square • Planted August 2007 m2 Tot.-N 3,4-3,8 P 0,6 K 0,9 Ca 1,5 Mg 0,2 Controlled experiment in phytotrone • 9 C • 15 C • 21 day/9 night • 21 12 hours light • 21 24 hours light • Rhodiola: clonal plants from Finland and Norway • Leuzea: clonal plants and seed-population, Finland • Serratula: seedpopulation Effect of temperature and day length on growth on Leuzea charthamoides Effect of temperature and day length on growth on Serratula coronata Effect of temperature and day length on growth on Rhodiola rosea mg/g dry weight Adaptogenic compounds in Rhodiola rosea 6 5 4 3 2 1 0 9 degrees 15 degrees Conclusions on growth • 12 hours days decrease growth to a large extent – all 3 species – most dramatic for Serratula • Serratula is the only herb with best growth at 21C and the one with very slow growth at 9C • Rhodiola grow best at low temperatures and tendence to prefer differentiated day/night temperatures • To early to say anything about the effects on adaptogens • Could be clonal differences – not time to analyse results in detail • Young plant material Kiitos, Takk, Spasiba, Thank you!