Transcript Alternative Management Techniques

Alternative Application
Technology
Daniel L. Kline
USDA-ARS, CMAVE
Gainesville, FL
[email protected]
Alternative ApplicationTechnology
• Area repellents
– Tent studies
• Insecticide-treated vegetation
– Semi-field: CMAVE protocol
– Field: Studies in Arkansas versus
Anopheles
Military Relevance
Pristine Sand Fly Habitat … Everywhere
Before Tent Cities
Army Tent City
Air Force Tent City
Area Repellents: Tent Studies
Metofluthrin (SumiOne™)
A highly volatile pyrethroid
F
O
F
O
MeO
F
F
Participants
• CMAVE
– Dan Kline, Jerry Hogsette and Brian Quinn
– Joyce Urban and Melissa Doyle
• NECE
– CDR Steve Rankin
– LCDR Dan Szumlas
– Dr. Todd Walker
• Valent
– John Lucas
Tent Studies: Metofluthrin (Sumi OneTM)
•
•
•
•
Types of emanators
Placement of emanators
Number of emanators
Environmental effects
30 ft wide
60 ft long
13’ 6” wide
TENT B
TENT A
100 feet
18’ 6” long
West Cage
East Cage
Tent configurations
SumiOne™ Paper Emanators
Surface area 4000 cm3 (both faces)
Treated with 200 mg SumiOne®
Placement of emanators
SumiOne™ Plastic Emanators
7.9 cm wide x 11 cm long (192
holes, each .25 cm2)
Plastic impregnated with 5% (w/w)
SumiOne®
Mosquito Magnets
and emanators
Mosquitoes Released
in Each Cage
Ochlerotatus taeniorhynchus (500)
Aedes aegypti (500)
Measures of Emanator Efficacy
• Landing counts on human volunteers (inside and
outside tent)
• Mosquito Magnet Pro collections (within tents)
• Residue analysis
% reduction
Percent reduction in trap collections
with SumiOne paper at two heights
100
90
80
70
60
50
40
30
20
10
0
60
19
30
8
40"
Inches above the ground
24 hours
48 hours
78"
Percent
Percent residue of metofluthrin
in paper emanators
100
90
80
70
60
50
40
30
20
10
0
78"
40"
0
24
Hours of exposure
48
Average percent reduction in trap collection
using SumiOne plastic emanator
100
90
% reduction
80
70
60
50
40
30
20
10
0
1
2
3
4
7
8
9
10 11 12 27 31 32 33 38 39 44 45 46 58 59 60
Days in tent
Inside
Outside
Untreated
Inside
Outside
Treated
Treated
Untreated
Nets
Overall Summary
1. SumiOne® shows potentially good knockdown
and bite inhibition activity against mosquitoes.
2. Type and placement of SumiOne emanators is
very important.
3. Further research is needed on type and
placement of emanators.
4. Additional candidate compounds need to be
evaluated.
Large outdoor screen cage (16’ high)
West
60’
Coleman
MD-2500
10’
10’
XXXXXXX
30’
XXXXXXX
South
XXXXXXX
North
XXXXXXX
East
Wax myrtle (Myrica cerifera)
7 plants per side
Participants
• CMAVE
– Dan Kline, Jerry Hogsette, Sandy Allan and
Brian Quinn
– Joyce Urban, Aaron Lloyd and Melissa Doyle
• Arkansas
– David Dame (Entomological Services)
– Max Meisch (University of Arkansas)
CMAVE Protocol
Mini-plots
Bifenthrin
residual
treatment
wax
myrtle
Bifenthrin
(Talstar) residual
treatment on Myricaon
cerifera
(wax myrtle).
Summer, 2005.
400
375
Plants + Talstar, no. dead
Plants + Talstar, no. alive
Plants with no Talstar, no. alive
350
No. of moquitoes captured
325
300
275
250
225
200
175
150
125
100
75
50
25
0
8/02
8/03
8/04
8/05
8/09
Date
8/16
8/23
8/30
9/07
Arkansas Studies
David Dame (Entomological Services) and Max Meisch, Univ. of Arkansas
Mini-plots (10’ x 10’)
0.1 acre plots
Bifenthrin .11 and .22 lb/acre
Bifenthrin .22 lb/acre
Trap Collections
Bifenthrin
treated mini-plots
Bifenthrin (Talstar One) treated mini plots.
Avg. trap capture
Summer 2006.
5000
4500
4000
3500
3000
2500
2000
1500
1000
500
0
1
2
3
Week
Control
Low tmt
High tmt
4
Bifenthrin
treated
plots
Bifenthrin (Talstar
One) treatedfield
field plots.
Avg. trap capture
Summer 2006.
5000
4500
4000
3500
3000
2500
2000
1500
1000
500
0
1
2
3
Week
Control
High tmt
Bioassay
% mortality
Percent mortality of An. quadrimaculatus
after 1 hr exposure to Bifenthrin-treated
leaves from field plots
100
90
80
70
60
50
40
30
20
10
0
1
2
Week
Control
High dose
3
% mortality
Percent mortality of An. quadrimaculatus
after 24 hour exposure to Bifenthrin
(Talstar) treated leaves from field plots.
100
90
80
70
60
50
40
30
20
10
0
1
2
3
Week
Control
High dose
% mortality
Percent mortality of Oc. taeniorhyncus
after one hour exposure to Bifenthrin
(Talstar) treated leaves from field plots.
100
90
80
70
60
50
40
30
20
10
0
1
2
Week
Control
High dose
3
Percent mortality of Oc. taeniorhyncus
after 24 hour exposure to Bifenthrin
(Talstar) treated leaves from field plots.
100
90
% mortality
80
70
60
50
40
30
20
10
0
1
2
Week
Control
High dose
3
Residue Analysis
Future Studies
Comparison of regular backpack sprayers
with electrostatic sprayers