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The influence of mycorrhiza on antioxidant enzymes activity and flavonoid synthesis
in Allium cepa cultivars.
Wężowicz K.a, Świadek M.a, Rąpała-Kozik M.b, Anielska T.a, Turnau K.a
a
b
Institute of Environmental Sciences, Jagiellonian University, Kraków,
Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University
INTRODUCTION
METHODS
Onion (Allium cepa) is one of the most consumed
vegetables worldwide and an important source of
valuable bioactive compounds with antioxidant activities.
Inoculation of plants with arbuscular mycorrhizal fungi
(AMF) is useful in areas poor in minerals.
Mycorrhizal and nonmycorrhizal plants were cultivated in sterile substratum under
greenhouse conditions. Flavonoid concentrations were determined by GC/MS. To
determine SOD activity photochemical reduction of NBT to formazan was used. The
activity of thiamine monophosphate synthase (TMPS) was characterized by HPLC.
Mycorrhizal colonisation was assessed according to Trouvelot method.
AIM OF STUDY
Evaluation of the effectiveness of mycorrhizal fungi in
cultivation of Polish onion varieties.
Flavonoid concentrations
RESULTS
Biomass
Onions
Significant increase in concentrations of quercetin, apigenin and kaempferol
were observed in mycorrhizal cvs. Wolska and Kuba.
Leaves
The highest increase in biomass due to the presence of mycorrhiza was found in cvs.
Kuba and Wolska.
Significant increase in concentrations of quercetin and kaempferol were
observed in leaves of mycorrhizal cvs. Wolska and Sochaczewska; Wolska also
showed higher apigenin concentrations. In leaves of nonmycorrhizal cv. Kuba
increase in flavonoid concentrations was found.
M+ : plants inoculated with G. intraradices
M- : noninoculated plants
Antioxidant activity
SOD activity
TMPS activity
16
U∙mg-1min-1
U∙g(FW)-1∙min-1
1
0.8
0.6
0.4
14
12
10
8
6
4
2
0.2
0
0
Wolska M-
M+
nmol(TMP)∙min-1∙g(FW)-1
1.2
nmol(TMP)∙min-1∙mg-1
1.4
Wolska M+
Ishikura M-
Ishikura M+
Wolska M-
Wolska M+
Ishikura M-
Ishikura M+
M-
M+
In mycorrhizal cvs. Wolska and Ishikura activity of SOD and TMPS increased in comparison to nonmycorrhizal plants.
Take home messages
 inoculation with G. intraradices results in significant biomass increase in plants;
 the effect of mycorrhiza inoculation on onion variety and its flovonoid concentrations depends on cultivar and environmental conditions;
 mycorrhizal inoculation stimulates activity of antioxidant enzymes.
PhD studies co-financed by the European Union under the European Social Fund
These investigations were carried out under financial support of the Ministry of Science and Higher Education, project 197/N-COST/2008/0.
M-