Transcript Document

Multiple phylogenetic species of morels (Morchella)
occur in landscape settings in the Pacific Northwest
McCotter, S.W., Aujla, I.S., Garfinkel, A.R., Jardini, T.M., Mullahy, S., Xia, C., Adams, M., Carris, L.M., & Peever, T.L.
Advanced Fungal Biology Class, Department of Plant Pathology, Washington State University, Pullman
Introduction
Morels (Morchella spp.) are popular and high-value edible ascomycetes composed of over 40 species that display a wide distribution throughout the northern
hemisphere (O’Donnell et al., 2011). Investigations utilizing advances in molecular species recognition techniques have shown that morel species are geographically
distinct which suggests that morels are not successful at colonizing new niche environments (O’Donnell et al., 2011). The limited research that has been conducted to
identify morels from cultivated landscape settings indicates that this ecological niche is dominated exclusively by two species: M. importuna and M. rufobrunnea (Kuo et
al., 2012; Mann and Mann, 2014). Most species of morels are described as having smooth spores, but studies by Malloch (1973) and Paraguey-Leduc et al (1998) suggest
that striately ornamented ascospores are a common feature in morels. The purpose of this project was 1) characterize the spore ornamentation of landscape morels
from the Pacific Northwest (PNW), and 2) test the hypothesis of niche restriction for morel species by determining the identity of landscape morels in the PNW.
Isolate
Code
Methods
Morels were collected in spring from suburban
landscapes (Table 1). Isolates were grown on
PDA amended with streptomycin-penicillin
covered by dialysis membranes. DNA was
extracted from lyophilized mycelium using a
modified phenol-chloroform protocol (Pagliaccia
et al., 2011). EF-1α, RPB1, RPB2 and nLSU rDNA
were PCR-amplified and Sanger-sequenced in
both directions using primers from O’Donnell et
al. (2011). The alignments for each locus were
concatenated. jModelTest 2.1.4 was used to
identify the TrNef+I model of nucleotide
substitution. MrBayes 3.2.2 and GARLI 0.96
were used for Bayesian and maximum likelihood
phylogenetic inference, respectively. Ascospores
were prepared using critical point drying for
Scanning electron microscopy (SEM) and
mounted in lactic acid for light microscopy (LM).
Collection
Year
Collection Location
1
Bark bed – Pullman, WA
2013
2
Landscape – Moscow, ID
2011
3
Landscape rock – Salt Spring Island, BC
2013
4
Landscape rock – Salt Spring Island, BC
2013
5
Landscape rock – Salt Spring Island, BC
2013
6
Bark bed – Pullman, WA
2013
7
Bark bed – Pullman, WA
2013
8
Bark bed – Pullman, WA
2013
9
Lawn – Pullman, WA
2011
10
Bark bed – Pullman, WA
2013
11
Lawn – Pullman, WA
2011
Table 1. Specimens used in study with collection
locations and collection year.
Results
• Landscape specimens represented 5 distinct species (Fig. 1), including 3 previously
reported from non-landscape settings
• All ascospores examined with LM and SEM were striate or wrinkled in
ornamentation (Fig. 1)
Fig. 1. Cladogram showing the identity of landscape morels (colored boxes); images of ascocarps, and
ascospores (SEM and LM) shown at right. Bolded lines represent bootstrap values and posterior probabilities
greater than 70.
Summary
We found that the 11 morels collected from landscape settings at 9 sites in the PNW represent five distinct species of morels (Fig. 1). Most of the specimens were M.
importuna, but we also identified three lineages previously unreported in landscape settings: M. brunnea, M. populiphila, and M. snyderi. These three species have only
been reported from forests and other undisturbed habitats (Kuo et al., 2012), demonstrating that there is overlap in ecological niches among different species of morels.
The fifth lineage we identified is Mel-8 (O’Donnell et al. 2011), a lineage that has only been identified once from a backyard in Northern California (Kuo et al. 2012). Our
Mel-8 specimen is the first with a mature ascocarp and ascospores. Ascospore ornamentation is striate or wrinkled in the 4 species we examined (Fig. 1). The
ornamentation is visible with both LM and SEM (Fig. 1), suggesting that spore ornamentation is not an artifact of SEM. Our results showing spore ornamentation is
consistent with previous studies by Malloch (1973) and Paraguey-Leduc et al. (1998). Additionally, three species of morels have been described with ornamented
spores (Chen and Liu, 2005; Elliot et al., 2014; Isiloglu et al., 2010). These results suggest that ascospore ornamentation is a common feature in Morchella.
References: Chen, J-Y and P-G Liu. 2005. Mycotaxon 93: 89-93. Elliot et al. 2014. Mycologia 106: 113-118. Isiloglu et al. 2010. Mycologia 102: 455-458. Kuo et al. 2012. Mycologia 104(5): 1159-1177. Mann, H.
and P. Mann. 2014. Omphalina 5(2): 11-12. Malloch, D. 1973. Can. J. Bot. 51: 1519-1520. O’Donnell et al. 2011. Fungal Genetics and Biology 48: 252-265. Pagliaccia et al. 2011. Mycologia 103: 969-982. PargueyLeduc et al. 1998. Cryptogamie, Bryol. Lichenol. 19 (2-3): 277-292.