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Microscopes
Microscopes
•
Microscopes are
devices that produce
magnified images of
things that are too small
to see with the unaided
eye
means “small” in
Greek, and skopmeans “see.”
– Micro-
Types of Microscopes
•
Light microscopes
produce magnified
images by focusing
visible light rays.
•
Electron microscopes
produce magnified
images by focusing
beams of electrons.
Properties of Microscopes
•
When we examine microscopes, we focus on two
properties:
– 1)
Magnification—how much larger can it make
an object appear compared to its actual size
– 2)
Sharpness—the clearness and crispness of
the image
Light microscopes
•
Light microscopes are the most
commonly used microscopes.
•
Light microscopes can produce
clear images of objects at a
magnification of 1000x.
•
Compound light microscopes
allow light to pass through the
specimen and use two lenses to
form an image.
Electron Microscopes
•
Electron microscopes can be
used to view objects that are
1000x smaller than those
visible under a light
microscope
•
Because these microscopes
don’t use light, the images
produced have no color
•
Additionally, electron
microscopes cannot be used
to examine living things
Body Tube
Ocular lens
(Eyepiece)
Nosepiece
Objectives
Arm
Stage
Stage Clips
Coarse Adjustment
Diaphragm
Light
Fine Adjustment
WHAT’S MY POWER?
•
To calculate the power of
magnification, multiply
the power of the ocular
lens (in the eyepiece) by
the power of the
objective lens
Comparing Powers of
Magnification
•
We can see better details with
higher the powers of
magnification, but we cannot
see as much of the image.
•
Which of these two images is
viewed at the higher power of
magnification?
Let’s give it a try ...
1 – Turn on the microscope and then rotate the nosepiece to
click the red-banded objective into place.
2 – Place a slide on the stage and secure it using the stage
clips. Use the coarse adjustment knob (large knob) to get it the
image into view and then use the fine adjustment knob (small
knob) to make it clearer.
3 – Once you have the image in view, rotate the nosepiece to
view it under different powers. Draw what you see on your
worksheet!
Be careful with the largest objective! Sometimes
there is not enough room and you will not be able to
use it!
4 – When you are done, turn off the microscope and put up the
slides you used.