Transcript Exploration and Purification of Bioactive compound from seaweeds

Exploration and Purification of
Bioactive compound from
seaweeds against human
bacterial pathogen
INTRODUCTION
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Most pathogenic bacteria becoming resistant to drugs, due
to indiscriminate use of antibiotics .
It becomes a greater problem of giving treatment against
resistant pathogenic
Seaweeds are macrophytic algae, a primitive type of plants
lacking true roots, stems and leaves.
Seaweeds are considered as the source of bioactive
compounds which act against both gram positive and gram
negative pathogenic bacteria.
Aim of the work
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Screening of seaweed extract which posses bioactivity against
human pathogens.
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To isolate and purify the compounds from seaweed extract
using silica column chromatography.
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To analyze the bioactivity of isolated pure compound.
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To identify the bioactive compound by Mass spectroscopy
and NMR.
MATERIALS
AND
METHODS
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Seaweeds were collected from Leepuram, Kootapuli
coastal area, Kanyakumari district.
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The seaweeds collected were identified and washed with
tap water followed by distilled water and air dry under
shady place for two weeks.
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Dried seaweeds are ground to 2mm or smaller particle size.
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1g of each seaweed powder are mixed with solvents in
shaker for 10 minutes. (methanol, chloroform, ethyl acetate
and hexane).
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The crude extract thus obtained was screened for its
bioactivity using the standard disc diffusion method.
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Incubated at 320 c for 24 hours.
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Formation of zone of inhibition indicate the antimicrobial
activity of the particular extract.
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The zone between 7 to 8 mm is excellent , 6 to 7 is good ,
less than that as poor.
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Purification of
column
bioactive compounds - silica
chromatography
and
Thin
layer
chromatography.
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Pure compound is obtained and anaylzed for its
bioactivity.
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Pure bioactive compound was identified in Mass
spectroscopy and NMR.
RESULTS
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The
seaweeds
collected
were
Amphiroa
foliaceae,
Chactomorpho tortuosa, Caulerpa scalpelliforms and Sargassum sp.
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The bioactivity of
seaweed extracts were determined
against Klebsilla sp, Staphylococcus aureus and proteus sp.
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The zone of inhibition was found in the Amphiroa foliacea
in solvent hexane and Sargassam sp in the solvent ethyl
acetate against Proteus sp, Staphylococcus aureus respectively.
Zone of inhibition 8 mm was found against
Staphylococcus aureus with Sargassam sp.
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The crude extract of Sargassam sp was selected for
purification of bioactive compound by Silica column
chromatography.
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The fraction 4 possessed a single compound in TLC.
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The single compound fraction 4 was analyzed for its
bioactivity.
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A zone of inhibition about 7mm was found against
Staphylococcus aureus
TLC of fraction 4
Antibacterial activity of fraction 4
ESIMS profile
MS/MS spectra of m/z
279 of Fraction 4
Future work
 Research
on Αlpha hydroxy stearic acid
could be carried out as lead compound
against the pathogen Staphylococcus aureus
Thank You