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EPCS Environmental LLC
Environmental Professional
Contracting Services
Dale Anderson
equipment
EPCS Environmental LLC July 1, 2008
Innovative solutions. Measurable results.
products
targets
EPCS Environmental LLC
Biofilm
equipment
New Health Risk
Innovative solutions. Measurable results.
products
targets
Kill Attacking Amoebae
ScienceDaily (July 25, 2008) — Bacteria rarely come as loners; more often they grow in crowds
and squat on surfaces where they form a community together. These so-called biofilms develop
on any surface that bacteria can attach themselves to. The dilemma we face is that neither
disinfectants and antibiotics, nor phagocytes and our immune system can destroy these biofilms.
 Amoebae normally attack and kill harmful pathogens
 Pathogens have become Resistant (biofilm bacteria use
chemical weapons to defend themselves.)
 They clump together and build a slimy fortress of defense
 Effective in the presence of all amoebae, killing them or causing
them to become de-activated
Common Problem Areas
 Athletic Facilities
 Hospitals
 Implants
 Catheters
 Surfaces
 Surgical equipment
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ÆGIS/mPale … Microbe Shield
 Microbe Shield Program based on a
unique antimicrobial technology which
effectively controls a broad spectrum of
bacteria, fungi, algae and yeasts on a
wide variety of treated substrates
 Used safely and effectively for 30 years
 Tested and approved for use in EPCS
electrostatics sprayers
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ÆGIS/mPale … Microbe Shield
The Mark of Excellence
FEATURES
BENEFITS
Non-Leaching
Does not migrate
Apply with Electrostatics Efficient and timely application process
Permanent Application
BONDS to surface
EPA Registered in 1976
Complies to strict Federal guidelines
SAFE
Renders many microorganisms lifeless via
an electrochemical process
Provides Water
Repellency
Intrinsic feature that helps preserve the
substrate
Non-Flammable
Safe for all surfaces
Odorless
Does not cause allergic reactions
Warranty
Available against mold/mildew growth
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ÆGIS/mPale Microbe Shield Treatment
Staphylococcus aureus ( MRSA )
Photographs taken by electronic microscope
Not Treated
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Treated
5
ÆGIS/mPale … Microbe Shield
Representative Microorganisms Tested:
A Partial Compendium
BACTERIA
Escherichia coli
Bacillus subtilis
Micrococcus sp.
Proteus mirabilis
Bacillus cereus
Staphylococcus epidermidis
Proteus mirabillis
Clostridium perfringens
Enterobacter agglomerans
Citrobacter diversus
Haemophilus influenzae
Acinetobacter calcoaceticus
Salmonella typhosa
Haemophilus suis
Staphylococcus aureus
(pigmented)
Salmonella choleraesuis
Lactobacillus casei
Staphylococcus aureus
(non-pigmented)
Corynebacterium bovix
Leuconostoc lactis
Klebsiella pneumoniae
ATCC 4352
Mycobacterium smegmatis
Listeria monocytogenes
Pseudomonas aeruginosa
Mycobacterium tuberculosis
Propionibacterium acnes
Pseudomonas aeruginosa
Brucella cania
Proteus vulgaris
Pseudomanas aeruginosa
PDR-10
Brucella abortus
Pseudomonas cepacia
Streptococcus faecalis
Brucella suis
Pseudomonas fluorescens
Escherichia coli ATCC 23266
Streptococcus mutans
Xanthomonas Campestris
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ÆGIS/mPale … Microbe Shield
Representative Microorganisms Tested:
A Partial Compendium (cont’d)
FUNGI
Mucor sp.
ALGAE
Aspergillus niger
Tricophyton
mentagrophytes
Oscillatoria borneti LB143
Aspergillus fumigatus
Tricophyton interdigitalie
Anabaena cylindrica B1446-1C
Aspergillus versicolor
Trichoderma flavus
Selenastrum gracile B-325
Aspergillus flavus
Chaetomium globusum
Pleurococcus sp. LB11
Aspergillus terreus
Rhizopus nigricans
Schenedesmus quadricauda
Penicillium Chrysogenum
Cladosporium herbarum
Gonium sp. LB 9c
Penicillium albicans
Aerobasidium pullulans
Volvox sp. LB 9
Penicillium citrinum
Fusarium nigrum
Chlorella vulgarus
Penicillium elegans
Fusarium solani
Penicillium funiculosum
Gliocladium roseum
YEAST
Penicillium humicola
Oospora lactis
Saccharomyces cerevisiae
Penicillium notatum
Stachybotrys atra
Candida albicans
Penicillium variabile
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The ÆGIS/mPale Microbe Shield
 Highly Effective
 Long-Lasting
 Odorless/Colorless
 Extremely Low Toxicity
 Long History of Safe Use
 Permanent Chemical Bonding
 Won’t Wash Off or Affect Skin Microflora
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mPerial™
 Cleaner, sanitizer and deodorizer
 Registered with the EPA as a germ killer and fungicide
and is authorized for use in food preparation areas. It is
specially formulated for compatibility with mPale
Antimicrobial.
 Virucide
 Fungicide (against pathogenic fungi)
 Mildewstat (on hard, inanimate surfaces)
 Effective in the presence of 5% serum contamination
 For home, hospital, institutional and industrial use,
school, restaurant, food handling and processing
areas
 Kills germs
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ÆGIS/mPale  mPerial™
Treatment
 All touch surfaces
• Fountain area for
drinks and ice
• Carpet and floor mats
• Doorknobs, handrails
• Rest rooms
• Play ground support
products
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ÆGIS/mPale  mPerial™
Treatment
 New construction
• Ceilings
• Floors
• Walls
• Wall cavities
• Above ceiling space
• Chases
• Furnishings
• A/C ducts
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ÆGIS/mPale  mPerial™
Treatment
 Transportation: Trucks
and Trailers
• Reduces bacteria load
• Reduces odors
• Makes treated surfaces
easier to clean
• Tremendous value to
cost ratio
• Tagging agent built-in to
verify validity
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EPCS Environmental LLC
Evaluation of a Silane
Quarternary Ammonium Salt
as Antimicrobial Fabric
Treatment
equipment
Arvye Davis, Randall Sumner, SLSTP-Flight Group2004
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Overview
Antimicrobial agents are used to kill
or limit growth of microorganisms.
ÆGIS
Microbe
Shield™,
an
antimicrobial agent composed of a
silane quaternary ammonium salt, is
featured for its durability and efficacy
against both gram-negative and
gram-positive bacteria. This research
studied
the effectiveness of the
antimicrobial agent as a treatment for
cotton fabric. Cotton fabric samples
were coated with the antimicrobial
agent, then tested against three
species of bacteria. The samples
were shaken in a bacterial solution to
allow contact with the microbes, and
the number of viable bacteria were
counted. The data were compared to
the numbers of bacteria with
untreated fabric. This research
reveals that the antimicrobial agent
reduced the numbers of bacteria
exposed to the treated cotton fabric.
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Research
 The three types of bacteria were
Escherichia coli, Staphylococcus
epidermidis, and Bacillus subtilus. For
each test, the bacteria were grown in
Tryptic Soy Broth (TSB) for eighteen
hours at room temperature. The
bacteria were diluted in 0.003M KH2PO4
to 72 percent transmission using a Vitek
Colorimeter. For testing, the bacteria
were diluted 1:1000 to 1.5-3.0 x 105
cfu/mL. Then, 50mL aliquots of the
diluted bacteria were distributed into
sterile 250mL flasks.
 The number of bacteria were
determined by the pour plate method
using Tryptic Soy Agar (TSA). The
control was plated at “0” contact time.
The nine flasks were agitated in a
reciprocal shaker at 120 RPM. After one
hour, all bacteria samples were plated
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and incubated for 24 hours at 35°C.
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300
It was discovered that the
antimicrobial agent did
exhibit the ability to kill the
three species of bacteria.
Present Bacteria CFU/mL (X 1000)
Results
250
200
150
100
50
0
E.coli
Staph
Treated
Untreated
Bacillus
Control
Graph 1: Comparison of all pure cultures used with and
without the antimicrobial agent.
Acknowledgements
This research was conducted as part of the 2004 Spaceflight and Life
Sciences Training Program funded by the National Aeronautics and Space
Administration. The authors recognize the support of the Dynamac
Corporation, the NASA Spaceflight and Life Sciences Training Program
Academic Partner Alliance and the United States Department of
Agriculture. I would like to thank my Principal Investigator, Randall Sumner,
for his hard work in preparation of this research, and I would also like to
thank Jason Johns and Adam Santone for their help and guidance in this
research.
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EPCS Environmental LLC
Electrostatic Spraying
Technology
equipment
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EPCS Environmental LLC
Inefficiencies of
Conventional Sprayers
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Inefficiencies of conventional
sprayers
 Of the total amount of product applied with
conventional sprayers
• 60% to 70% goes on the floor/ground
• 15% to 20% drifts from immediate area
• only 15 to 20% is deposited on the target
• less than 3% contacts near the intended area
under target
on target
drifts
floor/ground
floor/ground
drifts
on target
under target
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EPCS Environmental LLC
EPC
Advantages
equipment
Electrostatic Technology
Innovative solutions. Measurable results.
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Air-Assisted Electrostatic Sprayers
 The charged droplets seek out
surfaces by electrostatic forces. The
result is a uniform spray coverage in
hidden areas: underside, crevices,
holes, and empty spaces you can’t
see with a naked eye.
•RESULTS: is over 30 to 45-fold
increase to these hidden areas.
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Using electrical charges to
enhance the behavior of spray
OTHER BENEFITS:
Over 4 to 7-fold deposit efficiency improvement
Provides uniform dispersion and coating
Leaves no visible residue
Reduces “hang-time”
Coats air-borne and non-conductive targets
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BENEFIT: Reduces “Hang-Time”
1/30th of a second
Uncharged Spray
Charged Spray
Charged droplets move
much faster to the target
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BENEFIT: Reduces “hang-time”;
Coats air-borne and non-conductive targets
Demonstration of Electrostatic Coating
on Non-conductive Target
Uncharged spray
Charged spray
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EPCS Environmental LLC
Electrostatics Demo
“Black Ball”
equipment
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Black Ball Demo
First, we spray
the sphere with
electrostatics
turned OFF: 1 –
2–3–4
seconds.
(The target object was sprayed from a distance of
3 meters; the black background was added to
make the spray more visible.)
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Black Ball Demo
Notice the
coverage on the
target.
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Black Ball Demo
Finally, we turn
electrostatics
ON, and spray a
new sphere for
half the time: 1 –
2 seconds.
(All other settings are kept constant, so half the
amount of material is sprayed.)
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Black Ball Demo
Notice the
coverage on the
target. The
charged droplets
wrapped around,
reaching the
back of the
sphere.
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Black Ball Demo Results
Original Sphere
Electrostatics OFF
Electrostatics ON
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EPCS Environmental LLC
Successful Applications
Healthcare, Athletics/Sports Facilities, Food Industry,
Correctional Facilities, Airline Industry,
Schools/Colleges, Residential/Businesses
equipment
Innovative solutions. Measurable results.
products
targets
Control of Microorganisms in
Indoor Environments
EXAMPLES
 Medical Centers / Hospitals / Hospices
• DEMO
 Athletics / Sports Facilities
• Virginia Tech University
 Correctional Facilities
• Metro Detention Center
 Airline Industry
 Schools / Colleges
• Over 75 school coated in 2006 & 2007
 Residential / Businesses
• Mohawk - World’s largest floor manufacturer
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Case Study
Arthur G. James Cancer Hospital & Research Institute, Columbus, OH
Lee Memorial Hospital, Lee County, FL
Improved Control of Microbial
Exposure in Hospitals:
A 30 Month Field Study
© 2007 MaxSpray International Corporation
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Case Study
Washington Redskins, Washington, D.C.
Over 26 sports
facilities coating in
the last 22 months.
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Case Study
Metro Detention Center
Atlanta, GA
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Case Study
Airlines Industry
Test coating cabins of two 757’s
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Various Institutions:
 Virginia Tech
 SwathmoreCollage
 Ohio State University
 Atlanta Thrashers
 University of South
Carolina
 Cal State Bakersfield
 University of Tulsa
 Southern Illinois
University
 University of Illinois
Southern
 Stevens Institute of
Technology
 Columbia University
 Grundy Wrestling
 John Hopkins
University
 Missouri State
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Various Sports Facilities:
 Miami Heat
 New Jersey Nets
 Cleveland Browns
 Washington Redskins
 Washington Wizards  Minnesota Vikings
 Atlanta Hawks
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 Detroit Lions
38
The WOW F actors
 Electrostatic technology = The 1st Wow!
 mPale  mPerial products = The 2nd Wow!
 Ability to measure the amount of mPale
on the surface/target = The 3rd Wow!
$15M research was invested
to discover these Wows!
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Three Step Game Plan
 You need a plan designed to protect your
sports facilities, equipment and synthetic
turf fields.
 The big advantages to the Three Step
Game Plan
 Less Invasive
 Longer Lasting
 More Effective
 More Versatile
 Faster
 More Efficient
 Lower Cost
 Moisture Resistant
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Three Step Game Plan
Sophisticated Technologies
Advanced Application
Protocols
More Affordable Than Ever
What's your game plan?
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EPCS