Transcript Bacteria Lab - Aurora Public Schools

Bacteria Lab
Problem: Where is the biggest variation of
bacteria found within the school?
Background: Bacteria are found _________
They grow on nutrient agar, incubated at 37
degrees celsius.
Hypothesis:
Hypothesis: If we test for bacteria around
the school, then we will find the greatest
variety in ______________ because
_____________________
Procedure: (Don’t write)
1. Divide agar plate into 4 quadrants,
labeled 1, 2, 3, and 4. Do this on the
agar side of the plate (not the lid).
2. Put your name on the agar plate
1
2
3
4
name
3. Your agar plate is your pass to go into
the school to take samples. Do not
disrupt any classes or be rude to anyone
in the school.
4. Gently swab an area that you want to
test, and then gently swab the Q-tip on
one quadrant of the agar plate. Don’t
press hard. Leave # 4 unswabbed, it will
be your control. Use a new Q-tip for
each area that you swab. Don’t leave
your agar plate exposed to the air, as
bacteria are floating around in the air, as
well. Record your data in the data
section of your lab.
5. Return to class with your agar plate.
Seal the edges with masking tape…there
should be no gaps…why?
6. Check to make sure you have your name
on the agar plate, and that you have
each area recorded in your data section.
Put agar plate back in the tray, so I can
put it in the incubator.
7. Make sure your used Q-tips end up in
the garbage can. If I am sending you off
into the school on your own, the
assumption is that you will behave
appropriately!!
Data:
1.
2.
3.
4.
Draw what you see……
1
2
3
4 (control)
(location)
(location)
(location)
(control)
Data: (continued)
Quadrant 1
location
Number
of
different
looking
colonies
Total # of
colonies
2
3
4
Conclusion: (To be done after your data is
collected.)