Transcript Microbiology_intro_2016

Welcome to Microbiology!
Why Study Microbiology?
When will I EVER use this again in my life Mrs. Z?
EBOLA
CDC Chronology / Ebola

http://www.cdc.gov/vhf/ebola/outbreaks/
history/chronology.html
December 31, 2014 CDC announced:
USA Epidemic Levels
Meningitis Outbreak – 9/2012
The Swine Flu Virus
Chipotle Faces Another
Foodborne Illness Outbreak, This
Time In Boston
Do I have your attention yet? …
Not yet? … Have you heard of MRSA?
OK – enough… 
On to some paper work!
Classroom Rules
Micro / Forensics / Zoo
 Have a
positive
attitude!
Respect Each Other
Respect this Room & Building
NOTIFY US IMMEDIATELY - If there are
any marks on the desk – PLEASE
 If you don’t – you may get blamed for it
by the next student, in the next class.
 If there is an accident in lab, class or
damage to anything in this room – LET ME
KNOW – “Life Happens” – it’s OK! 

BE ON TIME! GET IN YOUR SEAT
BEFORE THE BELL RINGS! –
Especially 1st Block!
Get right to work!
No Sleeping in Class
Raise your hand when you have
a question
THINK / QUESTION / INQUIRE &
DOUBT - Ask questions! : Remember:
There is no such thing as a dumb
question.
Be Prepared for Class
Things can get confusing – Never hesitate to ask…
NO EATING OR DRINKING
IN CLASSROOM
AT ANY TIME
NO
CELL
PHONES!
“Each of you have an
assigned pocket
Dispose of waste properly
This is NOT
PROPER!
Do not get up in the middle of class to throw
something away or sharpen your pencil
SAFETY IS #1
Lab Safety

Safety equipment;
procedures… FIRE
DRILLS / Code RED
Know What all Signs & Symbols mean
Cleanliness is Safety
WASH HANDS EVERYDAY BEFORE
YOU LEAVE THE CLASSROOM
WASH LAB
TABLE WITH
BLEACH
BEFORE
AND
AFTER
USE
Follow the Kenston Handbook Rules
Microbiology
Micro = Small / Biology = “Study of Life”
 Microorganisms = too small to be seen
without a microscope
 Prokaryotic Cells = No nucleus
 Eukaryotic Cells = Have a true nucleus

Examples of Microorganisms

Kingdom Archaebacteria: the most ancient
of all living things.
 Three
types:
 thermoacidophiles
 methanogens
 Halophiles
Kingdom Eubacteria

The "true bacteria"
they are classified by
shape and Gram
Stain.

Shapes:



Coccus = Spherical
Bacillus = Rod Shaped
Spirillum = Spiral
Bacteria usually smaller than a
Eukaryotic Cell
Skin Cell
Bacterial Endospore
Bacterial Cell
Coccus or Round (sphere shaped)
Bacillus
The Spirals
There are a few other
Kingdoms we will consider …
Kingdom: Fungi

Eukaryotic - Classified by the type of
spores they use to reproduce with.
Some Fungi are “microscopic”
Athlete’s Foot; Ringworm and Penicillin (for example)
Kingdom Protista

Eukaryotic – Classified by how they move
(flagellum, cilia or pseudopodia)
Euglena (flagellum)
Paramecium (cilia)
Amoeba (pseudopodia)
Many Protists Cause Disease
Malaria
 African Sleeping
Sickness
 Giardia
 Toxoplasmosis


Often transmitted by
a “vector” (insect for
example)
Viruses = “poison”
Not in a Kingdom …
 Why not?

Viruses
They are parasitic
 They need a host to reproduce

 (the
virus hijacks the cells organelles – and
makes copies of itself)

They have DNA or RNA (not both)
AGAR
AGAR
 (ā'gär)
n.
Made from algae (seaweed – from the ocean).
 And as a stabilizer and thickener in many food
products.

Home

for bacteria
The bacteria do not EAT agar; it is a lattice work
for them to hang on
Petri Dish

(Glass or Plastic) – a container that we will
pour the agar into.
Terms:
Sterile = “clean” – no life
 Contaminated = “dirty” not sterile un-pure
 Inoculate = introduce an organism into
another (on agar in Petri dish for example)
 Pathogenic = Causes disease
 Autoclave = “Sterilizer or pressure
cooker”, equipment and agar put into
canister for at least 20 minutes at 250
degrees.

Coccus or Round (sphere shaped)
Bacillus
The Spirals
Bacterial Colonies on
Petri Dishes
Sample Petri Dish Results
Agar contains all of the following:
Beef Extract = Protein
 Yeast Extract = Growth Factors,
 Sucrose or Dextrose = Energy


Different types of agar will grow different
types of bacteria
Gram Stain
Antibiotic Testing