Transcript Microbiology Practical I

Microbiology Practical I
The following slides were contributed
by your fellow BIO 251 students…
Use side “” key to view slides…
Lab 1: Eukaryotes and Microscopy
Know the parts
of the
microscope…
Lab 1: Eukaryotes and Microscopy
Paramecium caudatum
Lab 1: Eukaryotes and Microscopy
Euglena gracilis
Lab 1: Eukaryotes and Microscopy
Amoeba proteus
Lab 1: Eukaryotes and Microscopy
Fungus – Saccharomyces cerevisiae
Lab 1: Eukaryotes and Microscopy
Cyanobacteria ~ Nostoc
~Bacteria slides~
Micrococcus luteus
~Bacteria Slides~
Bacillus subtilis
~Bacteria Slides~
Bacillus anthracis
~Bacteria Slides~
Rhodospirillum rubrum
Sorry not in focus…
~Bacteria Slides~
See other file for Gram Stain, Endospore, Acidfast,
and Capsule Staining…
[ Adobe Acrobat of Gram and Endospore ]
Some Endospore Staining ~
Streak Plate:
Know colony characteristics (color, elevation, margin, etc.)
Spread Plate vs. Streak Plate:
Streak Plate:
Spread Plate:
Mixed – Bacillus subtilis & Serratia marcescens
Practical I Unknown Tests:
~ Simple Stain
~ Gram Stain
~ Endospore Stain
~ Acid-Fast Stain (Demo)
~ Capsule Stain (Demo)
~ Motility (Hanging Drop, Wet Mount, Soft Agar Deep)
~ Obligate Aerobe, Facultative Anaerobe,
Aerotolerant Anaerobes, Microaerophiles
(Gas Pak, Eugon Agar, Thioglycollate Broth)
Gas Pak Anaerobic vs. Aerobic:
Dilution Problems:
Dilution Problems:
Bacterial Growth Curve:
Make sure you know
how to:
Use a
spectrophotometer
to measure
absorbance.
Produce a log graph
of the calculations.
Calculate generation
time from a growth
curve.