Transcript Document

Free DNA
Receptor for
DNA
Chromosome
bacteria
Two strains of Acinetobacter calcoaceticus:
One strain is resistant to streptomycin – resistant
strain
One strain is not resistant to streptomycin –
sensitive strain
1 hour @ 60°C
Live Resistant Strain
Lysing solution +
Live Resistant
strain
Lysing solution +
Killed Resistant
strain
Tryptic soy agar
Live
Sensitive
Live
Resistant
A
B
C
S
R
S+
KR
D
KR
S = Sensitive
Killed
Resistant
R = Resistant
K = Killed Resistant
Week one
growth check:
A
B
C
D
KR
S
Yes
R
Yes
S+
KR
No
Yes
Bacteria were then
transferred to a
plate containing
TSA with
Streptomycin:
Plate contains TSA
and Streptomycin:
S+
KR
S
A
R
B
C
Week two
growth check:
Yes
No
S
Yes
A
R
S+
KR
C
B
Conclusions:
1) Lysing did kill the resistant strain
2) The sensitive strain was sensitive to streptomycin
3) Genetic transformation did occur