Transcript Taming Cancer by Inducing Immunity via E. coli

Taming Cancer by Inducing
Immunity via E. coli
Presenter: Xu Nanfang
Cai Danfeng
Chen Yufei
 First, we need a E.coli that can escape
the immune system after infusing into the
blood
Protect E.coli from
Immune System
K1:O16 capsule
Idea from “bactoblood”,
UCBerkeley, iGEM2007
 polysialic acid is a frequent coating on
mammalian cells, the human immune system
does not recognize K1 as foreign
 Together, these surface modifications allow the
bacterium to avoid detection by the immune
system and should extend the serum half-life to
several hours rather than the less-than-5
minutes observed with rough strains
 Both of these gene clusters have been installed
into the genome of MC1061 in the course of
preparing our chassis strain, MC828U
Amazing O2
 First role of O2: to locate the cancer cells
 Study shows that
where there’s cancer
existing, oxygen
level is usually
significantly lower
than other parts of
the body.
 E. coli is anaerobic
 Second role of O2: to act as a signal for
the transcription of a series of factors.
A
a
Densitysensing
Oxygensensing
K1:O16
hydrolysis
A
inv
Petra Dersch and Ralph R.Isberg: A region of the
Yersinia pseudotuberculosis invasin
protein enhances integrin-mediated uptake into
mammalian cells and promotes self-association
Suicidal Component
 Make sure that E.coli kills oneself after
several generations of division
 Advantages:
 Problems:
 1. Dependent on the
immune system of
oneself
 1. The efficiency of
eliminating capsules
 2. In vivo vs. in vitro
 2. Detect cancer at
an early stage
 3. Cancer-specific