Transcript Slide 1

By: Destiny Sanchez
Efren Miranda
Mirna Rocha
QTL (Quantitative Trait Loci) are the
locations on chromosomes where the genes
that influence quantitative traits reside.
(Robert J. Brooker)
QTL
A Quantitative Trait is a
trait of a gene that can be
described with numbers.
Phenotype

HDL Cholesterol (High Density Lipoproteins)
LDL Cholesterol (Low Density Lipoproteins)
Atherosclerosis
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Heart attacks and Stroke are a result of
atherosclerosis
A build up of fat around arterial walls will
eventually lead to complete arterial blockage
Could result in death

To perform QTL analysis on inbred mice in
order to determine the effects of a high fat
diet on HDL concentrations and
Atherosclerosis susceptibility.
C57BL/6J (B6) X
129S1/SvImJ(129)
B6x129 (F1)
F2 Progeny

Fed high diet to 294 (F2) female mice for 14
weeks
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Measured plasma HDL concentrations

Measured size of aortic fatty-streak lesions
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Performed QTL analysis
Mice
n
Plasma HDL
Aortic Lesions
B6
10
41±2
951±459
129
10
106±4
0
F1
8
84±3
0
F2
294
81±1
228±44
129 mice had high HDL levels and
Atherosclerosis resistance
B6 mice displayed low HDL and High
Atherosclerosis susceptibility
F2 intercross - 6 Loci for HDL were
identified and 5 loci for heart disease
susceptibility
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
A high fat diet plays a large role in the
damaging of the aortic system leading to
heart disease.
The QTL for traits are located on the same
regions of the chromosomes in mice and
humans.
High fiber diet
Vegetables
Low Fat
Lean Protein
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
Brooker, Robert J.. Genetics Analysis and
Principles. New York, NY: McGraw-Hill,
2009.
http://beckerinfo.net/bioinformatics/?p=111
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Dr. Randy Von Smith
Tobias Beckwith
Dr. Brook Milligan
RISE Program