Transcript Biotechnology lentikats in food industry

Production of Invert Sugar
using Lentikats Biotechnology
Content
 Our aim
 What is Lentikats Biocatalyst
 Production of Lentikats Biocatalyst
 Benefits of Lentikats Biotechnology
 Lentikats Biocatalyst in Food Industry
 Our guarantees
 Our partners
 Contacts
LentiKat’s a.s. in Food Industry
 LentiKat’s a.s. follows up on nine year of research and development of Lentikats
Biotechnology.
 Our production know-how for commercial use of our Biocatalyst and for its industrial
applications is patented internationally.
 Our main focus is in intensification of production processes that leads to:
• higher process yields
• enhanced process stability,
• Increased revenue,
• Reduction of environmental footprint.
Biotechnologies are changing
the world…
Lentikats Biotechnology
Unique shape of the matrix and original immobilisation procedure enables to
encapsulate free enzymes or microorganisms (bacteria, yeasts, fungi) inside a
hydrogel porous carrier from polyvinyl alcohol (PVA). The advantages of PVA
matrix are:
• excellent physical and mechanical characteristics that result in a long-term
mechanical stability
• hardly biodegradable
• no toxic effects
Lentikats Biocatalysts enable easy separation of enzymes or microorganisms from
the bulk media and therefore can be used repeatedly.
With biotechnologies to
a better future…
How does the Lentikats Biocatalyst work?
A maximum surface in combination with the minimum volume of Lentikats
Biocatalyst results in a substantial reduction of diffusion limitations
associated with the transfer of substrate into the matrix and the product
out of the matrix.
Why polyvinylalcohol?
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It is an inexpensive material
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It is a non-toxic material
Encapsulation takes place under optimal, cell-sparing conditions
It allows a production of hydrogel with excellent mechanical properties
It does not have any side effects on fermentation
It offers high capacity for the cell loading
The process is stable and easy to control
Biotechnologies are changing
the world…
How is Lentikats Biocatalyst produced?
Lentikats Biocatalysts
• Biocatalyst Types for Use in Lentikats Encapsulation:
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Free Cells
• dead cells
• viable, non growing cells
• viable and growing cells
Free or Immobilised Enzymes.
Lentikats Biotechnology allows to immobilise any type of
enzyme
• The size of Lentikats Biocatalyst pores varies depending on the size
and diameter of the Biocatalyst due to:
• PVA additive type (size of macromolecule)
• additive concentration
• biomass concentration
Lentikats Biocatalysts
Manufacturing Plant
Laboratory Units for Encapsulation
• For low-capacity production of encapsulates (especially for research
purposes), a laboratory device is available
What are the benefits?
Matrix containing high
concentration of
biomass
Higher yields (in comparison to
conventional technologies using
free biomass)
Possibility of repeated
usage
Operational costs
savings
Enhanced resistance of
modified and
immobilised biomass
Higher process
stability
Easy separation of
biomass from reaction
media
Reduced costs for
downstream processes
(separation)
Lower production of
waste and by-products
Environmentally
friendly disposal
Higher
reaction
rates
Reduction of
reaction tanks’
volume
Operational costs
savings (electricity,
heat)
..and also…
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Shortening of the reaction times
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Much higher yields in comparison to conventional technologies (up to 5
– 15 % higher)
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Repetitive use of the Biocatalyst, easy separation from media (sieve
separator)
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Higher productivity of the Biocatalyst
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Lower risk of contamination during the production process
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High stability and easy control of production process
Biotechnologies are changing the world…
Technological set-up
 - inlet of reaction media
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 - medium with Lentikats Biocatalyst
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 - filling of Lentikats Biocatalyst
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 - stirrer
 - sieve separator
 - outlet of separated media
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 - outlet of Lentikats Biocatalyst
Lentikats and food industry
We currently offer solutions for the following processes:
 production of lactose free milk
Lentikats Biocatalyst with β-galaktosidase activity
 production of invert sugar
Lentikats Biocatalyst with invertase activity
 whey processing
Lentikats Biocatalyst with β-galaktosidase activity for:
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production of D-galaktose from whey lactose (pharmaceutical quality)
production of galacto-oligosaccharides from whey lactose
 production of special syrups
Lentikats Biocatalyst with invertase activity for production of glucose-fructose syrups
Lentikats Biocatalyst with immobilised glucoamylase for production of glucose from sugar
and starch-based materials or glucose syrups from maltotriose or maltose syrup
Lentikats and food industry
Following solutions are currently under development and testing:
 production of organic acids
Lentikats Biocatalyst with immobilised bacteria Bacillus coagulans for production of lactic
acid from lactose, glucose and sucrose
Lentikats Biocatalyst with immobilised filamentous fungi Aspergillus niger for production
of gluconic acid from sucrose and glucose
Lentikats Biocatalyst with immobilised filamentous fungi Aspergillus terreus for
production of itaconic acid from sucrose and glucose
Biotechnologies are changing the world…
Invert Sugar in Food Industry
@ Sucrose source – sugar beat, sugar cane
@ Mixture of glucose and fructose (sucrose hydrolysis)
@ Invert sugar as a product:
 composition 33% sucrose, 35% glucose and 32% fructose
» composition 4% sucrose, 51% glucose and 45% fructose
 invert sugar is more expensive than syrup
» invert sugar is sweeter and contains also other monosaccharides apart
from sucrose
 large variety of applications: kids food, pastry, soups and sauces, candy and
sweets, fruit drinks, dairy products.
Invert sugar in Distilleries
@ Sucrose source – sugar beat, sugar cane
@ Mixture of glucose and fructose
@ molasses – benefits of sucrose hydrolysis
 a longer shelf-life
» possibility of a year-round operation for molasses distilleries
 a lower water binding capacity – possibility to a achieve higher thickness
prior to storage (80%)
» lower demands for storage space and transportation
Lentikats Biotechnology and production
of invert sugar
Lentikats Biocatalyst with invertase activity
= immobilised saccharase (β-D-fructofuranosidase)
 saccharase source – Baker’s yeast (Candida utilis)
 invertase activity 600 - 1000 g sucrose/kg LB/hr
 optimum temperature 30 - 35°C
 optimum pH 4.5 – 5.0
 operational stability 600 – 1000 hodin
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Lentikats Biotechnology and production
of invert sugar
The active compound is immobilised invertase for sucrose hydrolysis to glucose and
fructose.
- Standard activity assay:
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batch test
standard sucrose solution with the concentration 100 g/l
temperature 30°C
pH 4.5 (10 mM acetate buffer)
conversion time 50 min
conversion rate 100%
Case studies
1) Hydrolysis of sucrose solution
Invert sugar production
Input parameters
 sucrose concentration 450 g/l
 pH = 4.5
 temperature = 30°C
 batch operation mode
 solution volume: 2 222 m3
Outlet
 concentration:
 sucrose 10 g/l
 glucose 220 g/l
 fructose 220 g/l
 volume 2 222 m3
Lentikats Biocatalyst
» activity 1000 g SUC/kg LB/hr
» stability 900 hr
» amount 1 ton
Invert sugar production
 amount 1 000 000 kg
14 EUR/m3 solution
 0.03 EUR/kg invert sugar
2) Hydrolysis of sucrose solution
Ethanol production
Input parameters
 sucrose concentration 450 g/l
 pH = 4.5
 temperature = 30°C
 batch operation mode
 Solution volume: 2222 m3
Lentikats Biocatalyst
» activity 1000 g SUC/kg LB/hr
» stability 1000 hr
» amount 1 ton
Output
 concentration:
 sucrose 10 g/l
 glucose 220 g/l
 fructose 220 g/l
 volume 2 222 m3
Ethanol production
 volume 615 333 litres a.a.
 14 EUR/m3 solution
 0.05 EUR/litre a.a.
3) Molasses distillery
Ethanol production
Input parameters
 sucrose concentration 120 g/l
 pH = 4.5
 temperature = 30°C
 continuous operation mode
 molasses volume: 8 333 m3
Output
 concentration:
 sucrose 30 g/l
 glucose 45 g/l
 fructose 45 g/l
 volume 8 333 m3
Lentikats Biocatalyst
» activity 1000 g SUC/kg LB/hr
» stability 1000 hr
» amount 1 ton
Ethanol production
 volume 629 167 litres a.a.
 4 EUR/m3 molasses
 0.05 EUR/litre a.a.
Summary – application of 1 ton of LB
Sucrose solution – 450 g/l sucrose
1 ton LB
Volume of solution
processed
[m3]
Ethanol
produced
[l a.a.]
Price
[EUR/m3 SUC
solution]
Price
[Kč/litr a.a.]
2222
615 333
14
0.05
Molasses – 120 g/l sucrose
1 ton LB
Volume of
molasses
processed
[m3]
Ethanol
produced
[l a.a.]
Price
[EUR/m3
molasses]
Price
[Kč/litr a.a.]
8333
629 167
4
0.05
Investment and Investment Return
Guarantees
•
You can easily verify our great results by testing our Biocatalyst in your or other
facilities with the material of your choice.
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Sample of Lentikats Biocatalyst free of charge
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Our company provides assistance and full technical support.
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Our company offers a long-term cooperation to companies, whose main concerns
are reduced investment costs and maximal process profitability.
Biotechnologies change the world…
Investment and Investment Return
Guarantees
We guarantee the following:

output parameters

operational
productivity
stability of Lentikats Biocatalyst and its
activity and
Our partners in the field of industrial
applications
Fermenta Biotech, DIL Complex, Ghodbunder
www.fermentabiotech.com
Road, Thane (West) - 400 610, Maharashtra, India
Protean, Pod Lesem 9, 373 16 Dobra Voda u Ceskych
Budejovic, Czech Republic
Pharmix s.r.o., Na Sadkach 2798, 767 01 Kromeriz,
Czech Republic
www.protean.cz
www.pharmix.cz
Our partners in the field of research and
development
Fermenta Biotech, DIL Complex, Ghodbunder Road,
Thane (West) - 400 610, Maharashtra, India
www.fermentabiotech.com
Institute of Microbiology,Academy of Science of the
Czech Republic
Vídeňská 1083, 142 20 Prague 4, Krč, Czech Republic
www.biomed.cas.cz
STU Bratislava, Fakulta chemické a potravinářské
technologie, Ústav biotechnologie a potravinářství,
Radlinského 9, 81237 Bratislava, Slovakia
www.stuba.sk
VŠCHT Praha, Ústav kvasné chemie a bioinženýrství,
Technická 5, 166 28 Praguea 6
www.vscht.cz
University of Alberta, Edmonton Alberta, T6G 2J9
Canada
www.ualberta.ca
Masaryk University, National Centre for Biomolecular
Research, Kamenice 5, 625 00 Brno-Bohunice
www.ncbr.chemi.muni.cz
Thank you for attention
Company Headquarters:
Office Building ARGO
LentiKat‘s a.s.
Evropska 846/176a
160 00 Prague 6
Czech Republic
Tel.:
Fax:
E-mail:
+ 420 255 710 680
+ 420 255 710 699
[email protected]
Production plant:
LentiKat‘s a.s.
Pod Vinicí 83
471 27 Stráž pod Ralskem
Czech Republic
Tel.:
Fax:
E-mail:
+ 420 487 851 393
+ 420 487 851 399
[email protected]
Laboratory:
INBIT LentiKat‘s a.s.
Kamenice 34
625 00 Brno
Czech Republic
Tel.:
Tel.:
+ 420 511 205 288
+ 420 511 205 287