Enzyme Inhibition

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Transcript Enzyme Inhibition

ENZYME INHIBITION
© 2008 Paul Billiet ODWS
Inhibitors
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Inhibitors are chemicals that reduce the rate of
enzymic reactions
The are usually specific and they work at low
concentrations
They block the enzyme but they do not
usually destroy it
Many drugs and poisons are inhibitors of
enzymes in the nervous system
© 2008 Paul Billiet ODWS
The effect of enzyme inhibition
Irreversible inhibitors: Combine with the
functional groups of the amino acids in the
active site, irreversibly
Examples: nerve gases and pesticides,
containing organophosphorus, combine with
serine residues in the enzyme acetylcholine
esterase
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© 2008 Paul Billiet ODWS
The effect of enzyme inhibition
Reversible inhibitors: These can be washed
out of the solution of enzyme by dialysis.
There are two categories
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© 2008 Paul Billiet ODWS
The effect of enzyme inhibition
1. Competitive: These
compete with the
substrate molecules for
the active site
The inhibitor’s action is
proportional to its
concentration
Resembles the substrate’s
structure closely
© 2008 Paul Billiet ODWS
E+I
Reversible
reaction
EI
Enzyme inhibitor
complex
The effect of enzyme inhibition
Fumarate + 2H++ 2e-
Succinate
Succinate dehydrogenase
CH2COOH
COOH
CHCOOH
CH2
CH2COOH
COOH
Malonate
© 2008 Paul Billiet ODWS
CHCOOH
The effect of enzyme inhibition
2. Non-competitive: These are not influenced by the
concentration of the substrate. It inhibits by binding
irreversibly to the enzyme but not at the active site
Examples
 Cyanide combines with the Iron in the enzymes
cytochrome oxidase
 Heavy metals, Ag or Hg, combine with –SH groups.
These can be removed by using a chelating agent such
as EDTA
© 2008 Paul Billiet ODWS
Applications of inhibitors
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Negative feedback: end point or end product
inhibition
Poisons snake bite, plant alkaloids and nerve
gases
Medicine antibiotics, sulphonamides,
sedatives and stimulants
© 2008 Paul Billiet ODWS
Enzyme pathways
Cell processes (e.g. respiration or photosynthesis) consist of
series of pathways controlled by enzymes
eB
eA
A
B
eC
C
eD
D
eF
E
F
Each step is controlled by a different enzyme (eA, eB, eC etc)
This is possible because of enzyme specificity
© 2008 Paul Billiet ODWS
End point inhibition
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The first step (controlled by eA) is often controlled
by the end product (F)
Therefore negative feedback is possible
A
eA
B
eB
C
eC
D
eD
E
eF
F
Inhibition
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The end products are controlling their own rate of
production
There is no build up of intermediates (B, C, D and E)
© 2008 Paul Billiet ODWS
Example: Phosphofructokinase and ATP
Substrate: Fructose-6-phosphate
Reaction
phosphofructokinase
fructose-6-phosphate + ATP  fructose-1,6-bisphosphate + ADP
© 2008 Paul Billiet ODWS
ATP is the end point
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This reaction lies near the beginning of the
respiration pathway in cells
The end product of respiration is ATP
If there is a lot of ATP in the cell this enzyme
is inhibited
Respiration slows down and less ATP is
produced
As ATP is used up the inhibition stops and the
reaction speeds up again
© 2008 Paul Billiet ODWS
The switch: Allosteric inhibition
Allosteric means “other site”
Active site
E
© 2008 Paul Billiet ODWS
Allosteric
site
Switching off
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These enzymes
have two receptor
sites
One site fits the
substrate like other
enzymes
The other site fits
an inhibitor
molecule
© 2008 Paul Billiet ODWS
Substrate
cannot fit into
the active site
Inhibitor
molecule
Inhibitor fits into
allosteric site
The allosteric site the enzyme “onoff” switch
Active
site
Substrate
fits into
the active
site
E
Allosteric
site empty
The inhibitor
molecule is
absent
© 2008 Paul Billiet ODWS
Conformational
change
Substrate
cannot fit
into the
active site
E
Inhibitor
molecule
is present
Inhibitor fits
into allosteric
site
A change in shape
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When the inhibitor is present it fits into its site
and there is a conformational change in the
enzyme molecule
The enzyme’s molecular shape changes
The active site of the substrate changes
The substrate cannot bind with the substrate
© 2008 Paul Billiet ODWS
Negative feedback is achieved
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The reaction slows down
This is not competitive inhibition but it is
reversible
When the inhibitor concentration diminishes
the enzyme’s conformation changes back to
its active form
© 2008 Paul Billiet ODWS
Phosphofructokinase
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This enzyme an active site for fructose-6-phosphate
molecules to bind with another phosphate group
It has an allosteric site for ATP molecules, the
inhibitor
When the cell consumes a lot of ATP the level of
ATP in the cell falls
No ATP binds to the allosteric site of
phosphofructokinase
The enzyme’s conformation (shape) changes and the
active site accepts substrate molecules
© 2008 Paul Billiet ODWS
Phosphofructokinase
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The respiration pathway accelerates and ATP (the
final product) builds up in the cell
As the ATP increases, more and more ATP fits into
the allosteric site of the phosphofructokinase
molecules
The enzyme’s conformation changes again and stops
accepting substrate molecules in the active site
Respiration slows down
© 2008 Paul Billiet ODWS