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Genetics News
Lost book
Mystery sequence project
Problem Day Friday
Unknown Day Monday
Where to get help before the exam
Wednesday, Sept 20
6:30 PM - 8:00 PM
E-107
Wednesday, Sept 20
7:00 PM - 9:00 PM
UFA2107 Jennifer Lee
Thursday, Sept 21
4:00 PM - 5:00 PM
S-215
Garrett Griffin
Friday, Sept 22
3:00 PM - 5:00 PM
S-215
Jeff Elhai
Sunday, Sept 24
8:00 PM - 10:00 PM
E-209
Karen Lewis
Monday, Sept 25
2:30 PM - 3:30 PM
S-111
Sarah Muller
Monday, Sept 25
5:15 PM - 6:15 PM
S-111
Sarah Muller
See Who We Are page on web.
Click on Special Help Sessions.
Sarah Muller
Pathways: Making Autos
Pathways: Making Autos
Pathways: Making Autos
Pathways: Making Autos
Pathways: Making Autos
Pathways: Making Autos
Pathways: Making Autos
substrate
Pathways: Making Autos
substrate
consumed
Product
Pathways: Making Pigment
Enzymes
Cell
chemical A
(vermilion color)
Pathways: Making Pigment
Enzyme X
Modify chemical A
Pathways: Making Pigment
Enzyme X
chemical B
(cinnabar color)
Pathways: Making Pigment
Enzyme Y
Modify chemical B
Pathways: Making Pigment
Enzyme Y
Wild-type pigment
(red color)
Pathways: Making Pigment
Accumulate
wild-type pigment
When Pathways Go Wrong
X+
Y+
Wild-type
eye color
X+
Y-
Cinnabar
eye color
X-
Y+
Vermilion
eye color
When Pathways Go Wrong
Imaginal disk tissue
XY+
Abdominal tissue
X+
Y-
When Pathways Go Wrong
Imaginal disk tissue
XY+
Wild-type
eye color
Abdominal tissue
X+
Y-
Pulse-Chase Experiment
Does RNA move from nucleus to cytoplasm?
Pre pulse
RNA
Uracil
U U U
U U
U U
U
U U U
U
U U
Pulse-Chase Experiment
Does RNA move from nucleus to cytoplasm?
Pulse
RNA
U
Uracil
U
U
U U
U U U
U
U U
U
U
U
U U
Pulse-Chase Experiment
Does RNA move from nucleus to cytoplasm?
Chase
RNA
Uracil
U U U
U U
U U
U
U U U
U
U U
Comparison of artificial RNA polymer
experiment & amino acid swap experiment
Experimental need
Need ribosomes, tRNA,
tRNA synthetases
Artificial polymer Amino acid swap
Cell extract
Cell extract
Need to focus on one
amino acid
Label each aa,
one at a time
Label cysteine
Need artificial polymer
Use poly(U,G)
Use poly(U,G)
Isolate new protein
Precipitate, filter
Precipitate, filter
Comparison of artificial RNA polymer
experiment & amino acid swap experiment
Experimental need
Need ribosomes, tRNA,
tRNA synthetases
Artificial polymer Amino acid swap
Cell extract
Cell extract
Need to focus on one
amino acid
Alter amino acid on
tRNA
Label each aa,
one at a time
Label cysteine
---
Convert cysteine
to alanine
Need artificial polymer
Use poly(U,G)
Use poly(U,G)
Isolate new protein
Precipitate, filter
Precipitate, filter
Classes of RNA and Why they are
Table 1. Comparison of classes of RNA in a typical bacterium (E. coli)
Class
Ribosomal RNA
(rRNA)
Types
Fraction
Sizes
2904bp,
1542bp, 120bp
Stability
3
80%
Transfer RNA
(tRNA)
37
12%
76bp to 91 bp
stable
Messenger RNA
(mRNA)
1000’s
5%
~300 bp to
~10,000 bp
unstable
stable
Function
Scaffold for ribosomal proteins,
participates in translation
Connects codon to amino acid
Carries information on amino acid
sequence of protein