A Biology and Engineering Cooperative Project

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Transcript A Biology and Engineering Cooperative Project

A Biology and
Engineering
Cooperative Project
James W. Stevens
M. Karen Newell
University of Colorado at Colorado
Springs
30 April 2005
general background
 connection
between cellular
metabolic pathways and immune
system response
 surface signals tied to mitochondrial
activity and fuel
cooperative effort

biology
– science driver
– cell cultures
 growing
 conditions
– cell counting and
physical preparation

engineering
– data acquisition
– temperature
measurements
– data reduction
– data smoothing
background-this project
2
mouse leukemia lines
L1210 – not drug resistant
L1210/ddp– drug resistant
 cultured
to 10^6 to 10^8 cells
 stressed with bleach
– measurable, repeatable differences
– different culture conditions, metabolic
states
cumulative heat release
L1210ddp
L1210
14
12
10
joules
8
6
4
2
0
16:51:36
16:52:19
16:53:02
16:53:46
-2
-4
time
16:54:29
16:55:12
16:55:55
data analysis
first law of thermodynamics:
dE
G  Q  W 
dt
dT
G  mc
 hA(T  T )
dt
measured heat generation rate
L1210ddp
L1210
6
5
4
watts
3
2
1
0
16:52:05
16:52:10
16:52:14
16:52:18
-1
-2
time
16:52:23
16:52:27
16:52:31
1
bt*
ae
y (t*) 
dt*
ce
0.5
0
0
0.2
0.4
0.6
0.8
1
data smoothing
least square curve fit:
minimize
 y(t*)  yˆ 
2
i
measured heat generation rate
L1210ddp, smoothed
L1210, smoothed
L1210ddp
L1210
6
5
4
watts
3
2
1
0
16:52:05
16:52:10
16:52:14
16:52:18
-1
-2
time
16:52:23
16:52:27
16:52:31
summary
 leverage
complementary capacities–
specific to people and project
 involve
students
 plunge
into a new area
other cooperative efforts at UCCS
 biomechanics
–biology, mechanical
engineering
 orthopedic mechanics –MERIC,
biology, mechanical engineering
 feedback controls –MAE, biology
 microgravity – MAE
 assistive technologies – MATH,
biology