Transcript poster_qMSF
Centre Hospitalier Universitaire Vaudois (CHUV) Faculté de Biologie et de Médecine de l’Université de Lausanne Quantitative Mass Spectrometry Facility (qMSF) Faculté de Biologie et de Médecine Dr. Bertrand Rochat, PhD, Project Leader, Head of Quantitative Mass Spectrometry Facility, BH18, CHUV, Rte du Bugnon, CH – 1011 Lausanne qMSF is a platform of mass spectrometer instruments of the Faculté de Biologie et de Médecine at CHUV. The platform is open to anyone who needs this technology. The Project Leader in charge of this facility is Dr. Bertrand Rochat The Goals of are : What can a Mass Spec do for YOU ?? to help all researchers who need mass spectrometers … Mass spectrometers are the best instruments to quantitate fundamental, to develop the own qMSF research … and identify drugs, endogenous molecules and proteins. biomedical, … in … and clinical At quantitate drugs, endogenous molecules and peptides research to perform routine analyses … mass spectrometers can : identify drug metabolites or peptide degradation products perform many other investigations Complementarity between the 2 MS facilities The Protein Analysis Facility at UNIL (www.unil.ch/paf) is the second open platform at UNIL and perform MS proteomic analysis : identification of proteins. to help partners in their research demanding MS technology What are LC-MS instruments ? liquid chromatography Some examples of expertise at qMSF to develop the own qMSF Research to perform routine analyses mass spectrometry • Quantification of antifungal drugs in patients’ plasma pump H+ N Liquid (H2O + CH3OH) + collision + injector m/z : 276.1 identification or/and quantification with specific softwares Intensity MS chromatogram Mass 207.1 3.7 min Pumps for vacuum samples Ion Source Mass Spectrometer I • Quantification of protease inhibitors in patients’ plasma 207.1 • Resistance to anti-cancer agents mediated by drug metabolism in cancer cells • Identification of peptide degradation by-products • Quantitation of antifungal drugs, etc… in patient plasma samples • Etc … 276.1 Precursor ion mass 0 100 Mass Spectrometer II 200 300 207.1 400 500 600 Product ion Mass Detection 26 ng/mL HN O O Analytical Column : separation of drugs or peptides • Quantification of NPY and other peptides in plasma mass 0 100 200 300 400 500 • Pharmacologie Clinique at CHUV Some partners … 600 • Maladie Infectieuse at CHUV • GKT SA at Geneva • Centre Intégratif de Génomique at Lausanne Time [min.] • Etc … For feasibility and evaluation, quadrupoles Q1, Q2 and Q3 Please, At qMSF, we have a Triple Quadrupole and a Linear Ion Trap MS instruments Ion source quadrupole Dr. Bertrand Rochat, Project Leader, Quantitative Mass Spectrometry Facility, FBM, BH18-228, CHUV, CH – 1011 Lausanne E-mail : [email protected] ; phone ++41 (0) 21 314 41 58