Transcript Microfluidics in Medical Applications

Microbubble Generation for
Medical Applications
Shirley Zhang
Faculty Mentor: Abraham Lee
Graduate Student: Kanaka Hettiarachchi
Overview
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Microfluidic devices for generating
bubbles – test for optimal
conditions
Create double layer bubbles to
contain lipid and oil surrounding gas
Test stability and attachment of
bubbles in vitro
Making Devices
U.V.
Photoresist
Mask
Silicon wafer
Develop
Microfluidic
channels
PDMS
Monolayer bubbles
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Components:
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Lipid
(DSPE/DPPEPEG2000)
Gas (PFC or N2)
Diameter:
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~15 um
ColorCam
10x magnification
Fluorescence
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DiI mixed into lipids
ColorCam
40x magnification
Double Layer Bubbles
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Components:
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FastCam
Lipid
Oil
Gas (PFC or N2)
No lipid shell –
no concentrated
fluorescence
around bubbles
Problems
Triacetin
DSPC/DSPE-PEG2K
N2
FastCam
Future Direction
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Successfully make stable double
layer bubbles
Microbubbles as a drug delivery
system in cell culture