Transcript Slide 1

Examination of the State Dependent Properties of WIN-55-212-2 on Spatial Learning and Memory in Rats in the Sand Maze
Ashley R. Smith and Gretchen Hanson Gotthard
Randolph-Macon Woman’s College
Lynchburg, VA 24503
Results
No differences in latency were exhibited between groups on Day 1; however, differences
emerged on the first day of training when rats received WIN-2. Overall quadrant preference
did not differ between groups, but a breakdown of quadrant preference by minutes revealed
poorer performance in the different state group than in the same state groups.
Introduction
Naturally occurring cannabinoids (e.g., THC) and synthetic cannabinoids (e.g., WIN-55-212-2), have
been linked with hippocampal function, and therefore, spatial learning and memory. Although a
number of studies have shown a relationship between cannabinoid administration and learning and
memory deficits (e.g., Lichtman, et al., 1995), no studies have explicitly examined the state dependent
properties of synthetic cannabinoids.
The sand maze was used in the present study, and is an appetitive open-field spatial task that may
serve as an alternative to the water maze in some studies. While the water maze requires rats to swim
in a pool of water to locate a hidden platform (Morris, 1981), the sand maze requires rats to dig in a
pool of sand to retrieve buried cereal rewards (Hanson, 2003). The sand maze may be a better
alternative for studies that aim to examine spatial behavior without the potential side effects of
aversive tasks (e.g., increased amygdala activity and/or fight or flight responses).
Acquisition
Latency (in seconds)
State dependent retention refers to the tendency of organisms to recall information better when in the
same “state” they were in during learning, than if they were in a different state during learning and
testing (Spear and Riccio, 1994). In fact, if cannabinoid administration produces state dependent
effects, then a memory impairment following cannabinoid administration may not be due to decreased
hippocampal function alone, but may be due to an altered state between acquisition and training or
testing. The critical test for state dependent retention is to return the subject to the original state to
determine if an inaccessible memory can be retrieved.
Figure 1
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Trial
The state dependent properties of the synthetic cannabinoid WIN-55-212-2 (WIN-2) were examined
in the present study using the sand maze. It was hypothesized that any deficit produced by WIN-2
during training would be diminished by returning the subject to the original learning state during
testing (i.e., no drug).
Day 1: Shaping
Day 2: Training
Day 3: Training
Trial 1: Exposed
Trial 3: Shallow Buried Trial 5: Buried (Medium)
Trial 2: Partially Buried Trial 4: Buried (Shallow) Trial 6: Buried (Deep)
Different State
(n=6)
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(n=5)
Same State
(n=9)
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Day 10: Testing
Probe Trial (No Reward
Buried in the Sand)
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Discussion
•All rats performed equally well during shaping.
•Rats in the same state groups preferred the correct quadrant more than rats in the different
state group.
•Rats in the different state group appeared to exhibit a place aversion for the correct quadrant.
•Robinson, et al., 2003
•Future research might examine the state dependent properties of WIN-2 with a lower dose of
the drug (e.g., 3 mg/kg) to reduce the possible aversiveness produced by larger doses.
No Drug/No Drug
References
Hanson, G.R. (2003). The sand maze: An appetitive alternative to the Morris water maze
(Doctoral dissertation, Kent State University, 2003). Dissertation Abstracts
International, 63, 4958.
Lichtman, A. H., Dimen, K. R., & Martin, B. R. (1995). Systemic or intrahippocampal
cannabinoid administration impairs spatial memory in rats.
Psychopharmacology,119(3),282-290.
Morris, R.G.M. (1981). Spatial localization does not require the presence of local cues.
Learning and Motivation, 12, 239-260.
Robinson, L., Hinder, L., Pertwee, R. G., & Riedel, G. (2003). Effects of Delta-9tetrahydrocannabinol and WIN-55,212-2 on place preference in the water maze in rats.
Psychopharmacology, 166, 40-50.
Spear, N. E. & Riccio, D. C. (1994). Memory: Phenomena and Principles. Boston: Allyn &
Bacon.
Figure 3
Probe Trial (1-week test)
Quadrant Preference
Table 1
Quadrant Preference
Method
Rats were handled prior to shaping. A three-day procedure was used with two trials per day, which
included shaping, training, and testing (see Table 1). Rats were randomly assigned to groups that
determined whether they were injected with WIN-2 (5.6 mg/kg) or VEH during training and testing
(see Table 1). Rats received intraperitoneal (I.P.) injections of WIN-2 (5.6 mg/kg) or VEH 30 minutes
prior to training and testing trials. Latency to find the reward was measured during acquisition.
Latency to dig and quadrant preference were measured during videotaped test trials.
Quadrant Preference
As can be seen in Figure 2, there were no differences in overall quadrant preference between
the groups [ F(2,14) = 1.239, p > .05]. However, as seen in Figure 3, a breakdown of
quadrant preference by minutes showed significant differences between the groups during
Minute 1 [ F(2,14) = 4.794, p < .05] and Minute 4 [F(2,14) = 4.231, p < .05]. An LSD post
hoc analysis showed that the different state group spent significantly less time (p < .05) in the
correct quadrant than the same state groups. The different state group showed a quadrant
preference that was significantly below chance during Minute 4 [t(5) = 3.043, p < .05] and
Minute 10 [t(5) = -2.632, p < .05] of the probe trial which would indicate a place aversion to
the correct quadrant.
Figure 2
Retention Probe Trial
Subjects
The subjects were 90-day old, male Long-Evans rats (N=20). Rats were reduced to and maintained at
85% of their free-feeding weights one week prior to and during the experiment. Water was available
ad libitum.
Apparatus
The sand maze was a plastic pool (36 inches wide by 6 inches deep) filled with a sand/crushed Froot
Loops (FL) cereal mixture that was 2 inches deep (approximately 11 ounces of FL was crushed and
mixed with 100 pounds of play sand to make the mixture). The maze was elevated 36 inches off the
floor.
Procedure
Each rat was placed in the sand maze and required to find FL, which were located in one consistent
location during shaping and training (i.e., NW, NE, SW, or SE quadrant). Rats were started from a
different location in the maze on each trial (i.e., N, S, E, or W). After finding the reward on any given
trial, the rat was allowed to consume approximately three to four FL prior to termination of the trial.
Acquisition
As can be seen in Figure 1, latency did not differ between groups on Day 1: Shaping [Trial 1,
F(2,14) = 0.150, p > .05, and Trial 2, F(2,14) = 1.146, p > .05]. Rats only differed on the first
day of drug administration [Trial 3, F(2,14) = 3.563, p = .05]. That is, rats receiving WIN-2
showed longer latencies to find the reward than rats receiving vehicle injections on the first
training trial.
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