PowerPoint Presentation - Cell Architecture: The Microtubule

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Transcript PowerPoint Presentation - Cell Architecture: The Microtubule

Organizing
the cell:
the
Cytoskeleton
Intermediate Filaments
Microfilaments
Microtubules
M
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o
t
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b
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e
s
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EM views
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FtsZ: a prokaryotic
ur-tubulin?
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The Tubulin Superfamily
alpha
Part of heterodimer
All Eukaryotes
beta
Part of heterodimer
All Eukaryotes
gamma
Part of microtubule
initiation complex
All Eukaryotes
delta
Centriolar component
(c-tubule)
Various
epsilon
Localized to centriole
in a cell-cycle specific
manner
Various
eta
Basal body component
Paramecium
zeta
Basal body component
Trypanosomes
FtsZ
Component of
filaments
Prokaryotes
Microtubule model
exchangeable
GDP
GTP
“Minus”
end
“Plus” end
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GTP
GDP
GDP
GDP
GDP
GDP
GDP
GDP
GDP
GTP
GTP
GTP GTP
GTP
GTP
GTP
GTP
GTP
GTP
GTP
GTP
GTP
GTP
GTP Beta-tubulin “cap”
GTP
Plus
In
Tubulin dimer
atomic structure
Out
Minus
Microtubule fly-by
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Narrated Video
Microtubule
initiation
MTOCs
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The Centrosome
revealed
9-triplet microtubule centrioles
pericentriolar material
singlet microtubules
Washout of
nocodazole
10 um
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Successive images at
2 minute intervals after
changing to drug-free
media (at timepoint A)
Dynamic Instability in vitro
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Video
Dynamic instability in vivo
Video
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Video
Fluorescence
Speckle
Microscopy
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Disassembly
Stathmin
microtubulebinding domain
Video
In vitro
assembly on
axonemal
microtubule
seeds
+/- Stathmin
Activity
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Katanin
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Video
Microtubule wall
(Mitotic Extract)
(Interphase Extract)
Centrosomal release
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Treadmilling
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Speckles
Photobleaching
MAPs
Note the crossbridges (arrows)
between
microtubules
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Plus end tracking proteins (+TIPS)
Video
Video
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um
GFP::EB1
Cy3 Tubulin
Interrelationship between
microtubules and microfilaments
Microtubule effects
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Video
Microfilament effects
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Video
Synthesis and Folding
Signal transduction
stathmin
cof B
cof C
abGTP
CCT
b
Rac
Integration with actin
networks
Cdc42
cof E
a
PAK
cof D
GEF?
cof A
Rho
abGDP
Tub;
CLIP-170, EB-1
mDia
Dynamic
Polymer
stathmin
catastrophic kinesins
katanin
severin (EF1a)
Pathways
Rho
IQGAP
stabilizing MAPs;
+ end capture
Stable
Polymer
Assembly/Disassembly