Transcript The Effect of Alcohol Solubility on Psuedomonas

The Effect of Alcohol Chain Length
on Psuedomonas Fluorescens Lag
Phase
BY: ASIA HILLIARD
SAM MCMILLAN
Introduction
 Pseduomonas fluorescens: a gram negative, rod-
shaped bacteria that is well-adapted to living in soil,
water, and plant surface environments.
Preliminary Experiment
Question & Methods
 Question: What is the effect of alcohols of varying
carbon chain length on the lag phase of
Pseudomonas fluorescens?
 Method
 Various alcohols
 Various concentration of alcohol
 Low salt Luria broth
 Distilled water
Results
Results
y = 778.93x - 697.5
R² = 0.98431
Conclusion
 The carbon chain length of alcohol is directly
proportional to an increase in Pseduomonas
fluorescens lag phase.
 Future Questions:
 To what extent does the trend line hold true?
 What causes propyl alcohols to have a different effect on
Pseudomonas fluorescens lag phase?
Resources
References
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Pugachev, Mikhail V.; Shtyrlin, Nikita V.;
Sapozhnikov, Sergey V.; et
al.Source: BIOORGANIC & MEDICINAL
CHEMISTRY Volume: 21 Issue: 23 Pag
es: 73307342 DOI: 10.1016/j.bmc.20
13.09.056 Published: DEC 1 2013
Yang, Shan; Zhang, Zhiqing; Wang, Fang;
et al.Source: JOURNAL OF
POLYMERRESEARCHVolume: 20Issue:
12 ArticleNumber: 307 DOI: 10.1007/s
1096501303071Published: NOV 2
2013
Lee, Soo Youn; Lee, Seonyoung; Lee,
Jiho; et al.Source: MATERIALS
LETTERS Volume: 110Pages: 229232
DOI: 10.1016/j.matlet.2013.08.050
Published: NOV 1 2013
Picture Sources
 http://www.online-
utility.org/image/gallery.
jsp?title=Alkanols
 http://prospect.rsc.org/b
logs/cw/2009/12/18/che
mistry-worlds-weeklyround-up-of-money-andmolecules-98/